LOCUS BBa_K404165 3751 bp DNA linear 03-Apr-2025 DEFINITION pCMV_VP1up_NLS_mVenus_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404165 VERSION BBa_K404165.1 FEATURES Location/Qualifiers misc_feature 2027..3628 /label=VP 3 misc_feature 2..655 /label=CMV misc_feature 673..1081 /label=VP 1 up misc_feature 1088..1108 /label=NLS misc_feature 1115..1828 /label=mVenus misc_feature 1835..3628 /label=VP 2 misc_feature 1835..3751 /label=AAV-2 cap (Stratagene) misc_feature 1835..3628 /label=VP 1 misc_feature 1947..2561 /label=AAP misc_feature 3182..3184 /label=HSPG-KO misc_feature 3173..3175 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg 721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca 781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg 841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc 901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc 961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt 1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaaggc 1081 gaccggccct gcaagaaaaa gattgaatac cggcatgagc aaaggcgaag aactgtttac 1141 cggcgtggtg ccgattctgg tggaactgga tggtgatgtg aacggccata aatttagcgt 1201 gagcggcgaa ggcgaaggtg atgcgaccta tggcaaactg accctgaaac tgatttgcac 1261 caccggcaaa ctgccggttc cgtggccgac cctggttacc accctgggct atggcctgca 1321 atgctttgcg cgttatccgg atcatatgaa acagcacgat ttctttaaaa gcgccatgcc 1381 ggaaggctat gtgcaggaac gcaccatctt ttttaaagat gatggcaact ataaaacccg 1441 tgcggaagtg aaatttgaag gcgataccct ggtgaaccgt attgaactga aaggcatcga 1501 tttcaaagaa gatggcaaca ttctgggcca taaactggaa tataactaca acagccataa 1561 cgtgtatatc accgcggata aacagaaaaa cggcatcaaa gcgaacttta aaatccgcca 1621 caacattgaa gatggcggcg tgcagctggc cgatcattat cagcagaaca ccccgattgg 1681 tgatggcccg gtgctgctgc cggataacca ttatctgagc taccagagca aactgagcaa 1741 agatccgaac gaaaaacgtg accatatggt gctgctggaa tttgtgaccg cggccggtat 1801 tacccatggc atggatgaac tgtataaaac cggcgctccg ggaaaaaaga ggccggtaga 1861 gcactctcct gtggagccag actcctcctc gggaaccgga aaggcgggcc agcagcctgc 1921 aagaaaaaga ttgaattttg gtcagactgg agacgcagac tcagtacctg acccccagcc 1981 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag 2041 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa 2101 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg 2161 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc 2221 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt 2281 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg 2341 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga 2401 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga 2461 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc 2521 agacgtcttc atggtgccac agtatggata cctcaccctg aacaacggga gtcaggcagt 2581 aggacgctct tcattttact gcctggagta ctttccttct cagatgctgc gtaccggaaa 2641 caactttacc ttcagctaca cttttgagga cgttcctttc cacagcagct acgctcacag 2701 ccagagtctg gaccgtctca tgaatcctct catcgaccag tacctgtatt acttgagcag 2761 aacaaacact ccaagtggaa ccaccacgca gtcaaggctt cagttttctc aggccggagc 2821 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg 2881 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa 2941 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa 3001 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc 3061 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac 3121 aaccaatccc gtggctacgg agcagtatgg ttctgtatct accaacctcc aggctggcaa 3181 cgcccaagca gctaccgcag atgtcaacac acaaggcgtt cttccaggca tggtctggca 3241 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca 3301 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct 3361 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc 3421 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctcca 3481 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc 3541 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg 3601 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt 3661 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga 3721 taagtagcat ggcgggttaa tcattaacta c //