LOCUS       BBa_K404166             3056 bp    DNA     linear       15-Jun-2024
DEFINITION  pCMV_VP1up_NLS_His-Tag_[AAV2]-VP23 (ViralBrick-587KO-Empty)
ACCESSION   BBa_K404166
VERSION     BBa_K404166.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    674..1082
                     /label=VP1up
     misc_feature    1089..1109
                     /label=NLS
     misc_feature    1116..1133
                     /label=His-tag
     misc_feature    1140..3056
                     /label=AAV-2-cap (Stratagene)
     misc_feature    1140..2933
                     /label=VP2
     misc_feature    1140..2933
                     /label=VP1
     misc_feature    1332..2933
                     /label=VP3
     misc_feature    2487..2489
                     /label=HSPG-KO
     misc_feature    2478..2480
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattagct
      661 agatggccgg cgctgccgat ggttatcttc cagattggct cgaggacact ctctctgaag
      721 gaataagaca gtggtggaag ctcaaacctg gcccaccacc accaaagccc gcagagcggc
      781 ataaggacga cagcaggggt cttgtgcttc ctgggtacaa gtacctcgga cccttcaacg
      841 gactcgacaa gggagagccg gtcaacgagg cagacgccgc ggccctcgag cacgacaaag
      901 cctacgaccg gcagctcgac agcggagaca acccgtacct caagtacaac cacgccgacg
      961 cggagtttca ggagcgcctt aaagaagata cgtcttttgg gggcaacctc ggacgagcag
     1021 tcttccaggc gaaaaagagg gttcttgaac ctctgggcct ggttgaggaa cctgttaagg
     1081 cgaccggccc tgcaagaaaa agattgaata ccggccatca tcaccatcac cacaccggcg
     1141 ctccgggaaa aaagaggccg gtagagcact ctcctgtgga gccagactcc tcctcgggaa
     1201 ccggaaaggc gggccagcag cctgcaagaa aaagattgaa ttttggtcag actggagacg
     1261 cagactcagt acctgacccc cagcctctcg gacagccacc agcagccccc tctggtctgg
     1321 gaactaatac gatggctaca ggcagtggcg caccaatggc agacaataac gagggcgccg
     1381 acggagtggg taattcctcg ggaaattggc attgcgattc cacatggatg ggcgacagag
     1441 tcatcaccac cagcacccga acctgggccc tgcccaccta caacaaccac ctctacaaac
     1501 aaatttccag ccaatcagga gcctcgaacg acaatcacta ctttggctac agcacccctt
     1561 gggggtattt tgacttcaac agattccact gccacttttc accacgtgac tggcaaagac
     1621 tcatcaacaa caactgggga ttccgaccca agagactcaa cttcaagctc tttaacattc
     1681 aagtcaaaga ggtcacgcag aatgacggta cgacgacgat tgccaataac cttaccagca
     1741 cggttcaggt gtttactgac tcggagtacc agctcccgta cgtcctcggc tcggcgcatc
     1801 aaggatgcct cccgccgttc ccagcagacg tcttcatggt gccacagtat ggatacctca
     1861 ccctgaacaa cgggagtcag gcagtaggac gctcttcatt ttactgcctg gagtactttc
     1921 cttctcagat gctgcgtacc ggaaacaact ttaccttcag ctacactttt gaggacgttc
     1981 ctttccacag cagctacgct cacagccaga gtctggaccg tctcatgaat cctctcatcg
     2041 accaatattt gtattacttg agcagaacaa acactccaag tggtaccacc acgcagagtc
     2101 gacttcagtt ttctcaggcc ggagcgagtg acattcggga ccagtctagg aactggcttc
     2161 ctggaccctg ttaccgccag cagcgagtat caaagacatc tgcggataac aacaacagtg
     2221 aatactcgtg gactggagct accaagtacc acctcaatgg cagagactct ctggtgaatc
     2281 cgggcccggc catggcaagc cacaaggacg atgaagaaaa gttttttcct cagagcgggg
     2341 ttctcatctt tgggaagcaa ggctcagaga aaacaaatgt ggacattgaa aaggtcatga
     2401 ttacagacga agaggaaatc aggacaacca atcccgtggc tacggagcag tatggatccg
     2461 tatctaccaa cctccaggct ggcaacgccc aagcagctac agctgatgtc aacacacaag
     2521 gcgttcttcc aggcatggtc tggcaggaca gagatgtgta ccttcagggg cccatctggg
     2581 caaagattcc acacacggac ggacattttc acccctctcc cctcatgggt ggattcggac
     2641 ttaaacaccc tcctccacag attctcatca agaacacccc ggtacctgcg aatccttcga
     2701 ccaccttcag tgcggcaaag tttgcttcct tcatcacaca gtactccacg ggacaggtca
     2761 gcgtggagat cgagtgggag ctccagaagg aaaacagcaa acgctggaat cccgaaattc
     2821 agtacacttc caactacaac aagtctgtta atgtggactt tactgtggac actaatggcg
     2881 tgtattcaga gcctcgcccc attggcacca gatacctgac tcgtaatctg taattgcttg
     2941 ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg gtctctgcgt atttctttct
     3001 tatctagttt ccatggctac gtagataagt agcatggcgg gttaatcatt aactac
//