LOCUS       BBa_K404168             3784 bp    DNA     linear       18-May-2024
DEFINITION  pCMV_VP1up_NLS_mVenus_VP2/3(ViralBrick-587-KO-His)
ACCESSION   BBa_K404168
VERSION     BBa_K404168.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    673..1081
                     /label=VP1up
     misc_feature    1115..1828
                     /label=mVenus
     misc_feature    1088..1108
                     /label=NLS
     misc_feature    1835..3661
                     /label=VP2
     misc_feature    2027..3661
                     /label=VP3
     misc_feature    3188..3205
                     /label=His-tag
     misc_feature    3182..3187
                     /label=TG-spacer
     misc_feature    3206..3214
                     /label=GLS-spacer
     misc_feature    3215..3230
                     /label=downstream 587
     misc_feature    3150..3181
                     /label=upstream 587
     misc_feature    1835..3784
                     /label=AAV-2-cap (Stratagene)
     misc_feature    3215..3217
                     /label=HSPG-KO
     misc_feature    3163..3165
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg
      721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca
      781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg
      841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc
      901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc
      961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt
     1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaaggc
     1081 gaccggccct gcaagaaaaa gattgaatac cggcatgagc aaaggcgaag aactgtttac
     1141 cggcgtggtg ccgattctgg tggaactgga tggtgatgtg aacggccata aatttagcgt
     1201 gagcggcgaa ggcgaaggtg atgcgaccta tggcaaactg accctgaaac tgatttgcac
     1261 caccggcaaa ctgccggttc cgtggccgac cctggttacc accctgggct atggcctgca
     1321 atgctttgcg cgttatccgg atcatatgaa acagcacgat ttctttaaaa gcgccatgcc
     1381 ggaaggctat gtgcaggaac gcaccatctt ttttaaagat gatggcaact ataaaacccg
     1441 tgcggaagtg aaatttgaag gcgataccct ggtgaaccgt attgaactga aaggcatcga
     1501 tttcaaagaa gatggcaaca ttctgggcca taaactggaa tataactaca acagccataa
     1561 cgtgtatatc accgcggata aacagaaaaa cggcatcaaa gcgaacttta aaatccgcca
     1621 caacattgaa gatggcggcg tgcagctggc cgatcattat cagcagaaca ccccgattgg
     1681 tgatggcccg gtgctgctgc cggataacca ttatctgagc taccagagca aactgagcaa
     1741 agatccgaac gaaaaacgtg accatatggt gctgctggaa tttgtgaccg cggccggtat
     1801 tacccatggc atggatgaac tgtataaaac cggcgctccg ggaaaaaaga ggccggtaga
     1861 gcactctcct gtggagccag actcctcctc gggaaccgga aaggcgggcc agcagcctgc
     1921 aagaaaaaga ttgaattttg gtcagactgg agacgcagac tcagtacctg acccccagcc
     1981 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag
     2041 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa
     2101 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg
     2161 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc
     2221 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt
     2281 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg
     2341 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga
     2401 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga
     2461 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc
     2521 agacgtcttc atggtgccac agtatggata cctcaccctg aacaacggga gtcaggcagt
     2581 aggacgctct tcattttact gcctggagta ctttccttct cagatgctgc gtaccggaaa
     2641 caactttacc ttcagctaca cttttgagga cgttcctttc cacagcagct acgctcacag
     2701 ccagagtctg gaccgtctca tgaatcctct catcgaccaa tatttgtatt acttgagcag
     2761 aacaaacact ccaagtggta ccaccacgca gagtcgactt cagttttctc aggccggagc
     2821 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg
     2881 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa
     2941 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa
     3001 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc
     3061 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac
     3121 aaccaatccc gtggctacgg agcagtatgg atccgtatct accaacctcc aggctggcaa
     3181 caccggccat catcatcatc atcatggcct gagcgcccaa gcagctacag ctgatgtcaa
     3241 cacacaaggc gttcttccag gcatggtctg gcaggacaga gatgtgtacc ttcaggggcc
     3301 catctgggca aagattccac acacggacgg acattttcac ccctctcccc tcatgggtgg
     3361 attcggactt aaacaccctc ctccacagat tctcatcaag aacaccccag tacctgcgaa
     3421 tccttcgacc accttcagtg cggcaaagtt tgcttccttc atcacacagt actccacggg
     3481 acaggtcagc gtggagatcg agtgggagct ccagaaggaa aacagcaaac gctggaatcc
     3541 cgaaattcag tacacttcca actacaacaa gtctgttaat gtggacttta ctgtggacac
     3601 taatggcgtg tattcagagc ctcgccccat tggcaccaga tacctgactc gtaatctgta
     3661 attgcttgtt aatcaataaa ccgtttaatt cgtttcagtt gaactttggt ctctgcgtat
     3721 ttctttctta tctagtttcc atggctacgt agataagtag catggcgggt taatcattaa
     3781 ctac
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