##gff-version 3
##sequence-region BBa_K404168 2 3784
BBa_K404168	.	sequence_feature	1088	1108	.	+	0	ID=annotation2095284;Name=NLS
BBa_K404168	.	promoter	2	655	.	+	0	ID=annotation2095279;Name=CMV
BBa_K404168	.	sequence_feature	1835	3661	.	+	0	ID=annotation2095285;Name=VP2
BBa_K404168	.	sequence_alteration	3163	3165	.	+	0	ID=annotation2101845;Name=HSPG-KO
BBa_K404168	.	sequence_feature	2027	3661	.	+	0	ID=annotation2095286;Name=VP3
BBa_K404168	.	sequence_feature	3182	3187	.	+	0	ID=annotation2095288;Name=TG-spacer
BBa_K404168	.	sequence_feature	3215	3230	.	+	0	ID=annotation2095292;Name=downstream 587
BBa_K404168	.	sequence_feature	3150	3181	.	+	0	ID=annotation2095293;Name=upstream 587
BBa_K404168	.	sequence_feature	3188	3205	.	+	0	ID=annotation2095287;Name=His-tag
BBa_K404168	.	sequence_feature	3206	3214	.	+	0	ID=annotation2095290;Name=GLS-spacer
BBa_K404168	.	sequence_feature	1115	1828	.	+	0	ID=annotation2095282;Name=mVenus
BBa_K404168	.	sequence_alteration	3215	3217	.	+	0	ID=annotation2101844;Name=HSPG-KO
BBa_K404168	.	sequence_feature	673	1081	.	+	0	ID=annotation2095281;Name=VP1up
BBa_K404168	.	sequence_feature	1835	3784	.	+	0	ID=annotation2095294;Name=AAV-2-cap (Stratagene)
>BBa_K404168
gcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagt
tcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgc
ccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatt
tacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaa
atggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatc
gctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc
tccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgc
cccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaaccc
actgcttactggcttatcgaaattactagatggccggcgctgccgatggttatcttccagattggctcgaggacactct
ctctgaaggaataagacagtggtggaagctcaaacctggcccaccaccaccaaagcccgcagagcggcataaggacgac
agcaggggtcttgtgcttcctgggtacaagtacctcggacccttcaacggactcgacaagggagagccggtcaacgagg
cagacgccgcggccctcgagcacgacaaagcctacgaccggcagctcgacagcggagacaacccgtacctcaagtacaa
ccacgccgacgcggagtttcaggagcgccttaaagaagatacgtcttttgggggcaacctcggacgagcagtcttccag
gcgaaaaagagggttcttgaacctctgggcctggttgaggaacctgttaaggcgaccggccctgcaagaaaaagattga
ataccggcatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacgg
ccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaactgatttgcaccacc
ggcaaactgccggttccgtggccgaccctggttaccaccctgggctatggcctgcaatgctttgcgcgttatccggatc
atatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatga
tggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgat
ttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcaccgcggata
aacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcggcgtgcagctggccgatcatta
tcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagc
aaagatccgaacgaaaaacgtgaccatatggtgctgctggaatttgtgaccgcggccggtattacccatggcatggatg
aactgtataaaaccggcgctccgggaaaaaagaggccggtagagcactctcctgtggagccagactcctcctcgggaac
cggaaaggcgggccagcagcctgcaagaaaaagattgaattttggtcagactggagacgcagactcagtacctgacccc
cagcctctcggacagccaccagcagccccctctggtctgggaactaatacgatggctacaggcagtggcgcaccaatgg
cagacaataacgagggcgccgacggagtgggtaattcctcgggaaattggcattgcgattccacatggatgggcgacag
agtcatcaccaccagcacccgaacctgggccctgcccacctacaacaaccacctctacaaacaaatttccagccaatca
ggagcctcgaacgacaatcactactttggctacagcaccccttgggggtattttgacttcaacagattccactgccact
tttcaccacgtgactggcaaagactcatcaacaacaactggggattccgacccaagagactcaacttcaagctctttaa
cattcaagtcaaagaggtcacgcagaatgacggtacgacgacgattgccaataaccttaccagcacggttcaggtgttt
actgactcggagtaccagctcccgtacgtcctcggctcggcgcatcaaggatgcctcccgccgttcccagcagacgtct
tcatggtgccacagtatggatacctcaccctgaacaacgggagtcaggcagtaggacgctcttcattttactgcctgga
gtactttccttctcagatgctgcgtaccggaaacaactttaccttcagctacacttttgaggacgttcctttccacagc
agctacgctcacagccagagtctggaccgtctcatgaatcctctcatcgaccaatatttgtattacttgagcagaacaa
acactccaagtggtaccaccacgcagagtcgacttcagttttctcaggccggagcgagtgacattcgggaccagtctag
gaactggcttcctggaccctgttaccgccagcagcgagtatcaaagacatctgcggataacaacaacagtgaatactcg
tggactggagctaccaagtaccacctcaatggcagagactctctggtgaatccgggcccggccatggcaagccacaagg
acgatgaagaaaagttttttcctcagagcggggttctcatctttgggaagcaaggctcagagaaaacaaatgtggacat
tgaaaaggtcatgattacagacgaagaggaaatcaggacaaccaatcccgtggctacggagcagtatggatccgtatct
accaacctccaggctggcaacaccggccatcatcatcatcatcatggcctgagcgcccaagcagctacagctgatgtca
acacacaaggcgttcttccaggcatggtctggcaggacagagatgtgtaccttcaggggcccatctgggcaaagattcc
acacacggacggacattttcacccctctcccctcatgggtggattcggacttaaacaccctcctccacagattctcatc
aagaacaccccagtacctgcgaatccttcgaccaccttcagtgcggcaaagtttgcttccttcatcacacagtactcca
cgggacaggtcagcgtggagatcgagtgggagctccagaaggaaaacagcaaacgctggaatcccgaaattcagtacac
ttccaactacaacaagtctgttaatgtggactttactgtggacactaatggcgtgtattcagagcctcgccccattggc
accagatacctgactcgtaatctgtaattgcttgttaatcaataaaccgtttaattcgtttcagttgaactttggtctc
tgcgtatttctttcttatctagtttccatggctacgtagataagtagcatggcgggttaatcattaactac