LOCUS BBa_K404222 4410 bp DNA linear 22-Nov-2024 DEFINITION [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-His-Tag) ACCESSION BBa_K404222 VERSION BBa_K404222.1 FEATURES Location/Qualifiers misc_feature 2..1867 /label=Rep78 misc_feature 2..1933 /label=Rep68 misc_feature 673..1933 /label=Rep40 misc_feature 673..1867 /label=Rep52 misc_feature 1883..4114 /label=VP1 misc_feature 2294..4114 /label=VP2 misc_feature 2489..4114 /label=VP3 misc_feature 4246..4409 /label=p5TATAless misc_feature 1883..4237 /label=AAV-a-cap (Stratagene) misc_feature 3245..3262 /label=His-tag misc_feature 3242..3244 /label=A-spacer misc_feature 3263..3265 /label=A-spacer ORIGIN 1 atggcggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc 61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat 121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctccag 181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg 241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg 301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt 361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc 421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa 481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg 541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag 601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact 661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag 721 cagtggattc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg 781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc 841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa 901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc 961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag 1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc 1081 aatgagaact ttcccttcaa cgactgtgtg gacaagatgg tgatctggtg ggaggagggg 1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc 1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc 1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg 1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag 1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg 1441 gagcatgagt tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca 1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg 1561 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg 1621 aatctgatgc tgtttccctg tagacaatgc gagagaatga atcagaactc aaatatctgc 1681 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt 1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg 1801 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa 1861 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga 1921 cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac caccaccaaa 1981 gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt acaagtacct 2041 cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg ccgcggccct 2101 cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt acctcaagta 2161 caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt ttgggggcaa 2221 cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg gcctggttga 2281 ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc ctgtggagcc 2341 agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa gattgaattt 2401 tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac agccaccagc 2461 agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac caatggcaga 2521 caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt gcgattccac 2581 atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa 2641 caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca atcactactt 2701 tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc acttttcacc 2761 acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga gactcaactt 2821 caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga cgacgattgc 2881 caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc tcccgtacgt 2941 cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct tcatggtgcc 3001 acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct cttcatttta 3061 ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta ccttcagcta 3121 cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc tggaccgtct 3181 catgaatcct ctcatcgacc aatatttgta ttacttgagc agaacaaaca ctccaagtgg 3241 tgctcatcat catcatcatc atgctaccac cacgcagagt cgacttcagt tttctcaggc 3301 cggagcgagt gacattcggg accagtctag gaactggctt cctggaccct gttaccgcca 3361 gcagcgagta tcaaagacat ctgcggataa caacaacagt gaatactcgt ggactggagc 3421 taccaagtac cacctcaatg gcagagactc tctggtgaat ccgggcccgg ccatggcaag 3481 ccacaaggac gatgaagaaa agttttttcc tcagagcggg gttctcatct ttgggaagca 3541 aggctcagag aaaacaaatg tggacattga aaaggtcatg attacagacg aagaggaaat 3601 caggacaacc aatcccgtgg ctacggagca gtatggatcc gtatctacca acctccagag 3661 aggcaacaga caagcagcta cagctgatgt caacacacaa ggcgttcttc caggcatggt 3721 ctggcaggac agagatgtgt accttcaggg gcccatctgg gcaaagattc cacacacgga 3781 cggacatttt cacccctctc ccctcatggg tggattcgga cttaaacacc ctcctccaca 3841 gattctcatc aagaacaccc cggtacctgc gaatccttcg accaccttca gtgcggcaaa 3901 gtttgcttcc ttcatcacac agtactccac gggacaggtc agcgtggaga tcgagtggga 3961 gctccagaag gaaaacagca aacgctggaa tcccgaaatt cagtacactt ccaactacaa 4021 caagtctgtt aatgtggact ttactgtgga cactaatggc gtgtattcag agcctcgccc 4081 cattggcacc agatacctga ctcgtaatct gtaattgctt gttaatcaat aaaccgttta 4141 attcgtttca gttgaacttt ggtctctgcg tatttctttc ttatctagtt tccatggcta 4201 cgtagataag tagcatggcg ggttaatcat taactactac tagagggagg ggtggagtcg 4261 tgacgtgaat tacgtcatag ggttagggag gtcctgtatt agaggtcacg tgagtgtttt 4321 gcgacatttt gcgacaccat gtggtctcgc tggggggggg ggcccgagtg agcacgcagg 4381 gtctccattt tgaagcggga ggtttgaact //