LOCUS BBa_K404242 3022 bp DNA linear 26-Dec-2024 DEFINITION pCMV_[AAV2]VP123 (ViralBrick-453-His-Tag) ACCESSION BBa_K404242 VERSION BBa_K404242.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 671..2899 /label=VP1 misc_feature 1079..2899 /label=VP2 misc_feature 1274..2899 /label=VP3 misc_feature 671..3022 /label=AAV-2-cap (Stratagene) misc_feature 1989..2026 /label=upstrem 453 misc_feature 2030..2047 /label=His Tag misc_feature 2051..2064 /label=downstream 453 misc_feature 1194..1808 /label=AAP ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg 721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca 781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg 841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc 901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc 961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt 1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac 1081 ggctccggga aaaaagaggc cggtagagca ctctcctgtg gagccagact cctcctcggg 1141 aaccggaaag gcgggccagc agcctgcaag aaaaagattg aattttggtc agactggaga 1201 cgcagactca gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct 1261 gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc 1321 cgacggagtg ggtaattcct cgggaaattg gcattgcgat tccacatgga tgggcgacag 1381 agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaacc acctctacaa 1441 acaaatttcc agccaatcag gagcctcgaa cgacaatcac tactttggct acagcacccc 1501 ttgggggtat tttgacttca acagattcca ctgccacttt tcaccacgtg actggcaaag 1561 actcatcaac aacaactggg gattccgacc caagagactc aacttcaagc tctttaacat 1621 tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag 1681 cacggttcag gtgtttactg actcggagta ccagctcccg tacgtcctcg gctcggcgca 1741 tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct 1801 caccctgaac aacgggagtc aggcagtagg acgctcttca ttttactgcc tggagtactt 1861 tccttctcag atgctgcgta ccggaaacaa ctttaccttc agctacactt ttgaggacgt 1921 tcctttccac agcagctacg ctcacagcca gagtctggac cgtctcatga atcctctcat 1981 cgaccaatat ttgtattact tgagcagaac aaacactcca agtggtgctc atcatcatca 2041 tcatcatgct accaccacgc agagtcgact tcagttttct caggccggag cgagtgacat 2101 tcgggaccag tctaggaact ggcttcctgg accctgttac cgccagcagc gagtatcaaa 2161 gacatctgcg gataacaaca acagtgaata ctcgtggact ggagctacca agtaccacct 2221 caatggcaga gactctctgg tgaatccggg cccggccatg gcaagccaca aggacgatga 2281 agaaaagttt tttcctcaga gcggggttct catctttggg aagcaaggct cagagaaaac 2341 aaatgtggac attgaaaagg tcatgattac agacgaagag gaaatcagga caaccaatcc 2401 cgtggctacg gagcagtatg gatccgtatc taccaacctc cagagaggca acagacaagc 2461 agctacagct gatgtcaaca cacaaggcgt tcttccaggc atggtctggc aggacagaga 2521 tgtgtacctt caggggccca tctgggcaaa gattccacac acggacggac attttcaccc 2581 ctctcccctc atgggtggat tcggacttaa acaccctcct ccacagattc tcatcaagaa 2641 caccccggta cctgcgaatc cttcgaccac cttcagtgcg gcaaagtttg cttccttcat 2701 cacacagtac tccacgggac aggtcagcgt ggagatcgag tgggagctcc agaaggaaaa 2761 cagcaaacgc tggaatcccg aaattcagta cacttccaac tacaacaagt ctgttaatgt 2821 ggactttact gtggacacta atggcgtgta ttcagagcct cgccccattg gcaccagata 2881 cctgactcgt aatctgtaat tgcttgttaa tcaataaacc gtttaattcg tttcagttga 2941 actttggtct ctgcgtattt ctttcttatc tagtttccat ggctacgtag ataagtagca 3001 tggcgggtta atcattaact ac //