LOCUS       BBa_K404243             3031 bp    DNA     linear       15-Jan-2026
DEFINITION  pCMV_[AAV2]VP123 (ViralBrick-453-RGD)
ACCESSION   BBa_K404243
VERSION     BBa_K404243.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    671..2908
                     /label=VP1
     misc_feature    1079..2908
                     /label=VP2
     misc_feature    1274..2908
                     /label=VP3
     misc_feature    1720..3031
                     /label=AAV-2-cap (Stratagene)
     misc_feature    1989..2026
                     /label=upstream 453
     misc_feature    2027..2029
                     /label=A-spacer
     misc_feature    2030..2056
                     /label=RGD
     misc_feature    2060..2073
                     /label=downstream 453
     misc_feature    2057..2059
                     /label=A-spacer
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg
      721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca
      781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg
      841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc
      901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc
      961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt
     1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac
     1081 ggctccggga aaaaagaggc cggtagagca ctctcctgtg gagccagact cctcctcggg
     1141 aaccggaaag gcgggccagc agcctgcaag aaaaagattg aattttggtc agactggaga
     1201 cgcagactca gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct
     1261 gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc
     1321 cgacggagtg ggtaattcct cgggaaattg gcattgcgat tccacatgga tgggcgacag
     1381 agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaacc acctctacaa
     1441 acaaatttcc agccaatcag gagcctcgaa cgacaatcac tactttggct acagcacccc
     1501 ttgggggtat tttgacttca acagattcca ctgccacttt tcaccacgtg actggcaaag
     1561 actcatcaac aacaactggg gattccgacc caagagactc aacttcaagc tctttaacat
     1621 tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag
     1681 cacggttcag gtgtttactg actcggagta ccagctcccg tacgtcctcg gctcggcgca
     1741 tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct
     1801 caccctgaac aacgggagtc aggcagtagg acgctcttca ttttactgcc tggagtactt
     1861 tccttctcag atgctgcgta ccggaaacaa ctttaccttc agctacactt ttgaggacgt
     1921 tcctttccac agcagctacg ctcacagcca gagtctggac cgtctcatga atcctctcat
     1981 cgaccaatat ttgtattact tgagcagaac aaacactcca agtggtgctt gcgattgccg
     2041 tggcgattgc ttttgcgcta ccaccacgca gagtcgactt cagttttctc aggccggagc
     2101 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg
     2161 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa
     2221 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa
     2281 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc
     2341 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac
     2401 aaccaatccc gtggctacgg agcagtatgg atccgtatct accaacctcc agagaggcaa
     2461 cagacaagca gctacagctg atgtcaacac acaaggcgtt cttccaggca tggtctggca
     2521 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca
     2581 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct
     2641 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc
     2701 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctcca
     2761 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc
     2821 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg
     2881 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt
     2941 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga
     3001 taagtagcat ggcgggttaa tcattaacta c
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