LOCUS BBa_K404252 2998 bp DNA linear 15-Nov-2024 DEFINITION pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Empty) ACCESSION BBa_K404252 VERSION BBa_K404252.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV promoter misc_feature 671..2875 /label=VP1 misc_feature 671..2998 /label=AAV-2-cap (Stratagene) misc_feature 1079..2875 /label=VP2 misc_feature 1274..2875 /label=VP3 misc_feature 2397..2428 /label=upstream 587 misc_feature 2429..2444 /label=downstream 598 misc_feature 2429..2431 /label=HSPG-KO misc_feature 2419..2421 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg 721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca 781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg 841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc 901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc 961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt 1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac 1081 ggctccggga aaaaagaggc cggtagagca ctctcctgtg gagccagact cctcctcggg 1141 aaccggaaag gcgggccagc agcctgcaag aaaaagattg aattttggtc agactggaga 1201 cgcagactca gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct 1261 gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc 1321 cgacggagtg ggtaattcct cgggaaattg gcattgcgat tccacatgga tgggcgacag 1381 agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaacc acctctacaa 1441 acaaatttcc agccaatcag gagcctcgaa cgacaatcac tactttggct acagcacccc 1501 ttgggggtat tttgacttca acagattcca ctgccacttt tcaccacgtg actggcaaag 1561 actcatcaac aacaactggg gattccgacc caagagactc aacttcaagc tctttaacat 1621 tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag 1681 cacggttcag gtgtttactg actcggagta ccagctcccg tacgtcctcg gctcggcgca 1741 tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct 1801 caccctgaac aacgggagtc aggcagtagg acgctcttca ttttactgcc tggagtactt 1861 tccttctcag atgctgcgta ccggaaacaa ctttaccttc agctacactt ttgaggacgt 1921 tcctttccac agcagctacg ctcacagcca gagtctggac cgtctcatga atcctctcat 1981 cgaccaatat ttgtattact tgagcagaac aaacactcca agtggtacca ccacgcagag 2041 tcgacttcag ttttctcagg ccggagcgag tgacattcgg gaccagtcta ggaactggct 2101 tcctggaccc tgttaccgcc agcagcgagt atcaaagaca tctgcggata acaacaacag 2161 tgaatactcg tggactggag ctaccaagta ccacctcaat ggcagagact ctctggtgaa 2221 tccgggcccg gccatggcaa gccacaagga cgatgaagaa aagttttttc ctcagagcgg 2281 ggttctcatc tttgggaagc aaggctcaga gaaaacaaat gtggacattg aaaaggtcat 2341 gattacagac gaagaggaaa tcaggacaac caatcccgtg gctacggagc agtatggatc 2401 cgtatctacc aacctccagg ctggcaacgc ccaagcagct acagctgatg tcaacacaca 2461 aggcgttctt ccaggcatgg tctggcagga cagagatgtg taccttcagg ggcccatctg 2521 ggcaaagatt ccacacacgg acggacattt tcacccctct cccctcatgg gtggattcgg 2581 acttaaacac cctcctccac agattctcat caagaacacc ccggtacctg cgaatccttc 2641 gaccaccttc agtgcggcaa agtttgcttc cttcatcaca cagtactcca cgggacaggt 2701 cagcgtggag atcgagtggg agctccagaa ggaaaacagc aaacgctgga atcccgaaat 2761 tcagtacact tccaactaca acaagtctgt taatgtggac tttactgtgg acactaatgg 2821 cgtgtattca gagcctcgcc ccattggcac cagatacctg actcgtaatc tgtaattgct 2881 tgttaatcaa taaaccgttt aattcgtttc agttgaactt tggtctctgc gtatttcttt 2941 cttatctagt ttccatggct acgtagataa gtagcatggc gggttaatca ttaactac //