LOCUS       BBa_K404253             3031 bp    DNA     linear       22-Dec-2024
DEFINITION  pCMV_[AAV2]-VP123ex (ViralBrick-587KO-His-Tag)
ACCESSION   BBa_K404253
VERSION     BBa_K404253.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    671..2908
                     /label=VP1
     misc_feature    1079..2908
                     /label=VP2
     misc_feature    1274..2908
                     /label=VP3
     misc_feature    671..3031
                     /label=AAV-2-cap (Stratagene)
     misc_feature    2435..2452
                     /label=His-tag
     misc_feature    2429..2434
                     /label=TG-spacer
     misc_feature    2453..2461
                     /label=GLS-spacer
     misc_feature    2397..2428
                     /label=upstream 587
     misc_feature    2462..2477
                     /label=downstream 587
     misc_feature    2454..2456
                     /label=HSPG-KO
     misc_feature    2420..2422
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg
      721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca
      781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg
      841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc
      901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc
      961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt
     1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac
     1081 ggctccggga aaaaagaggc cggtagagca ctctcctgtg gagccagact cctcctcggg
     1141 aaccggaaag gcgggccagc agcctgcaag aaaaagattg aattttggtc agactggaga
     1201 cgcagactca gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct
     1261 gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc
     1321 cgacggagtg ggtaattcct cgggaaattg gcattgcgat tccacatgga tgggcgacag
     1381 agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaacc acctctacaa
     1441 acaaatttcc agccaatcag gagcctcgaa cgacaatcac tactttggct acagcacccc
     1501 ttgggggtat tttgacttca acagattcca ctgccacttt tcaccacgtg actggcaaag
     1561 actcatcaac aacaactggg gattccgacc caagagactc aacttcaagc tctttaacat
     1621 tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag
     1681 cacggttcag gtgtttactg actcggagta ccagctcccg tacgtcctcg gctcggcgca
     1741 tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct
     1801 caccctgaac aacgggagtc aggcagtagg acgctcttca ttttactgcc tggagtactt
     1861 tccttctcag atgctgcgta ccggaaacaa ctttaccttc agctacactt ttgaggacgt
     1921 tcctttccac agcagctacg ctcacagcca gagtctggac cgtctcatga atcctctcat
     1981 cgaccaatat ttgtattact tgagcagaac aaacactcca agtggtacca ccacgcagag
     2041 tcgacttcag ttttctcagg ccggagcgag tgacattcgg gaccagtcta ggaactggct
     2101 tcctggaccc tgttaccgcc agcagcgagt atcaaagaca tctgcggata acaacaacag
     2161 tgaatactcg tggactggag ctaccaagta ccacctcaat ggcagagact ctctggtgaa
     2221 tccgggcccg gccatggcaa gccacaagga cgatgaagaa aagttttttc ctcagagcgg
     2281 ggttctcatc tttgggaagc aaggctcaga gaaaacaaat gtggacattg aaaaggtcat
     2341 gattacagac gaagaggaaa tcaggacaac caatcccgtg gctacggagc agtatggatc
     2401 cgtatctacc aacctccagg ctggcaacac cggccatcat catcatcatc atggcctgag
     2461 cgcccaagca gctacagctg atgtcaacac acaaggcgtt cttccaggca tggtctggca
     2521 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca
     2581 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct
     2641 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc
     2701 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctcca
     2761 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc
     2821 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg
     2881 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt
     2941 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga
     3001 taagtagcat ggcgggttaa tcattaacta c
//