##gff-version 3
##sequence-region BBa_K407014 1 3121
BBa_K407014	.	engineered_region	1	1069	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K407014	.	terminator	1029	1069	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K407014	.	engineered_region	1029	1069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K407014	.	stop_codon	1062	1062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K407014	.	polyA_site	1056	1069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K407014	.	stem_loop	1036	1055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K407014	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K407014	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K407014	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K407014	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K407014	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K407014	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K407014	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K407014	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K407014	.	CDS	227	907	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K407014	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K407014	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K407014	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K407014	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K407014	.	terminator	941	1020	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K407014	.	stem_loop	952	995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K407014	.	engineered_region	941	1020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K407014	.	engineered_region	1078	3121	.	+	0	ID=BBa_I13263;Name=BBa_I13263
BBa_K407014	.	promoter	2181	2235	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_I13263
BBa_K407014	.	promoter	2200	2205	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K407014	.	engineered_region	2181	2235	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K407014	.	start_codon	2233	2233	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K407014	.	promoter	2222	2227	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K407014	.	non_covalent_binding_site	2181	2200	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K407014	.	CDS	1255	2010	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I13263
BBa_K407014	.	point_mutation	1746	1746	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K407014	.	engineered_region	1255	2010	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K407014	.	engineered_region	1255	1256	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K407014	.	CDS	1255	2004	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K407014	.	point_mutation	1428	1428	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K407014	.	engineered_tag	2011	2035	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K407014	.	CDS	2262	2984	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I13263
BBa_K407014	.	terminator	2993	3072	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13263
BBa_K407014	.	stem_loop	3004	3047	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K407014	.	engineered_region	2993	3072	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K407014	.	promoter	1078	1228	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_I13263
BBa_K407014	.	start_codon	1205	1205	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K407014	.	engineered_region	1078	1228	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K407014	.	promoter	1166	1171	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K407014	.	non_covalent_binding_site	1207	1226	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K407014	.	non_covalent_binding_site	1078	1097	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K407014	.	promoter	1192	1199	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K407014	.	ribosome_entry_site	1237	1248	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13263
BBa_K407014	.	conserved_region	1241	1244	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K407014	.	terminator	3081	3121	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13263
BBa_K407014	.	engineered_region	3081	3121	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K407014	.	stop_codon	3114	3114	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K407014	.	polyA_site	3108	3121	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K407014	.	stem_loop	3088	3107	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K407014	.	terminator	2044	2123	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13263
BBa_K407014	.	stem_loop	2055	2098	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K407014	.	engineered_region	2044	2123	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K407014	.	ribosome_entry_site	2244	2255	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13263
BBa_K407014	.	conserved_region	2248	2251	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K407014	.	terminator	2132	2172	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13263
BBa_K407014	.	engineered_region	2132	2172	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K407014	.	stop_codon	2165	2165	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K407014	.	polyA_site	2159	2172	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K407014	.	stem_loop	2139	2158	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K407014
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgtt
aagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttt
taatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaaca
taaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatc
tgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatt
tcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtag
attattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgt
aattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatg
cttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattg
ttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaa
agaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtc
actttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacag
gagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaa
atggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcacc
ggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcg
atgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgac
caccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatg
cccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcg
agggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagct
ggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatc
cgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgc
tgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcct
gctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata