BBa_K408000 1 BBa_K408000 GFP with signal peptide for hemolysin system 2010-11-05T12:00:00Z 2015-05-08T01:12:25Z Created from chimeric PCR using BBa_E0840 and the hemolysin determinant from the plasmid pANN202-812 obtained from the Karolinska Instituet, Solna Sweden. The determinant is the same as present in pHly152 which was created from wild type E.coli. This GFP has been fused with the signal sequence for the alpha-hemolysin system to enable this peptide to be secreted by all E.coli and even other species with the generic trans membrane protein complex made by proteins coded by genes hlyB and hlyD. false false _523_ 0 6477 9 It's complicated false Apart from creating the fusion so that the stop codon is avoided and the frame does not shift, one of the Biobrick restriction sites PstI is also present in the part. false Pavan Choppakatla annotation2114290 1 Signal peptide range2114290 1 767 953 BBa_K408000_sequence 1 tctagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaatgcattagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagctgcaggtagcttcgatgttaaagaggaaagaactgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataactagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z