BBa_K411001 1 BBa_K411001 Theophylline-inducible Riboswitch 2010-10-14T11:00:00Z 2015-05-08T01:12:25Z ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaag forward primer : gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag reverse primer : ctgcagcggccgctactagtacttgttgtcttgcagcggggtgctgccaagggcatcaagac The discovery of the riboswitch was based on data which described conserved RNA secondary structure found on 5???-untranslated regions and the creation of small-molecule binding mRNA, aptamers. The function of these riboswitches is similar to the function of inducible promoters in that they both regulate downstream genetic data: their difference is that while promoters regulate transcription of DNA, riboswitches control translation of RNA. A riboswitch is can be separated into two components: an aptamer and an expression platform. These two components work together to form a ???switch???. The aptamer binds to a small molecule inducer, and the expression platform structurally changes to regulate gene expression. false false _526_ 0 6642 9 It's complicated true We want to choose a kind of riboswitch which has the following characteristics: The inducer cannot metabolize in E. coli. It does not exist in naturally occurring E. coli It does not have EcoRI,X,S, or PstI cutting site false Po-Hsiang Liao annotation2086256 1 Theophylline binding site (part B) range2086256 1 25 30 annotation2086255 1 rbs range2086255 1 46 56 annotation2088805 1 Theophylline binding site (part A) range2088805 1 10 11 BBa_K411001_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z