LOCUS       BBa_K411003             1127 bp    DNA     linear       22-Dec-2024
DEFINITION  pLAC promotor,Theophylline-inducible Riboswitch, GFP+Terminator
ACCESSION   BBa_K411003
VERSION     BBa_K411003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             254..264
                     /label=rbs
     misc_feature    233..238
                     /label=Theophylline binding site (part B)
     misc_feature    218..219
                     /label=Theophylline binding site (part A)
     misc_feature    209..264
                     /label=BBa_K411001
     CDS             271..990
                     /label=GFP protein
     CDS             271..990
                     /label=BBa_E0040
     stem_loop       1010..1053
                     /label=stem_loop
     misc_feature    999..1078
                     /label=BBa_B0010
     terminator      999..1078
                     /label=BBa_B0010
     stem_loop       1094..1113
                     /label=T7 TE
     misc_feature    1120..1120
                     /label=stop
     polyA_site      1114..1127
                     /label=polya
     misc_feature    1087..1127
                     /label=BBa_B0012
     terminator      1087..1127
                     /label=BBa_B0012
     misc_feature    271..1127
                     /label=BBa_J04630
     misc_feature    209..1127
                     /label=BBa_K411002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg tgataccagc atcgtcttga tgcccttggc
      241 agcaccccgc tgcaagacaa caagtactag atgcgtaaag gagaagaact tttcactgga
      301 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      361 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      421 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      481 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      541 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      601 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      661 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      721 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      781 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      841 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      901 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      961 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//