LOCUS       BBa_K411111             1229 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040+ B0034 + split_GFP_A+linker+split_eIF4A_A
ACCESSION   BBa_K411111
VERSION     BBa_K411111.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     CDS             81..551
                     /label=GFP_A
     CDS             552..581
                     /label=GSSGSGSGS linker
     CDS             582..1226
                     /label=eIF4A_A
     misc_feature    1227..1229
                     /label=stop
     CDS             81..1229
                     /label=BBa_K411101
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aggcagcagc ggcagcagcg gcagcggcag catggagccg gaaggcgtca
      601 tcgagagtaa ctggaacgag attgtggata gctttgatga catgaatctc tcagagtccc
      661 tcctccgtgg tatttatgcc tatggttttg agaagccctc tgccatccag cagcgagcta
      721 ttcttccttg tatcaagggt tatgatgtga ttgctcaagc ccagtctggg actgggaaaa
      781 cagctacatt tgccatatca attctccagc agattgaatt agatctaaag gccactcagg
      841 ctttggttct ggcacccaca cgtgaattgg ctcagcagat acaaaaggtg gttatggcat
      901 taggagacta catgggtgcc tcttgtcatg cctgcattgg gggcaccaat gtgcgtgctg
      961 aggtgcagaa gctccagatg gaagctcccc atatcatcgt gggtacccct ggccgggtgt
     1021 ttgacatgct taaccggaga tacctgtccc ccaaatacat caagatgttc gtactggatg
     1081 aagcagatga aatgttaagc cgagggttca aggatcagat ctatgacata ttccagaagc
     1141 tcaacagcaa cacacaggta gttttgttgt ctgctacaat gccttctgat gtccttgagg
     1201 tgaccaagaa atttatgaga gacccttga
//