LOCUS       BBa_K411121             1286 bp    DNA     linear       28-Jun-2024
DEFINITION  split_GFP_A+linker+split_eIF4A_A + terminator
ACCESSION   BBa_K411121
VERSION     BBa_K411121.1  
FEATURES             Location/Qualifiers
     CDS             1..471
                     /label=GFP_A
     CDS             472..501
                     /label=GSSGSGSGS linker
     CDS             502..1146
                     /label=eIF4A_A
     misc_feature    1147..1149
                     /label=stop
     CDS             1..1149
                     /label=BBa_K411101
     stem_loop       1169..1212
                     /label=stem_loop
     misc_feature    1158..1237
                     /label=BBa_B0010
     terminator      1158..1237
                     /label=BBa_B0010
     stem_loop       1253..1272
                     /label=T7 TE
     misc_feature    1279..1279
                     /label=stop
     polyA_site      1273..1286
                     /label=polya
     misc_feature    1246..1286
                     /label=BBa_B0012
     terminator      1246..1286
                     /label=BBa_B0012
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aggcagcagc
      481 ggcagcagcg gcagcggcag catggagccg gaaggcgtca tcgagagtaa ctggaacgag
      541 attgtggata gctttgatga catgaatctc tcagagtccc tcctccgtgg tatttatgcc
      601 tatggttttg agaagccctc tgccatccag cagcgagcta ttcttccttg tatcaagggt
      661 tatgatgtga ttgctcaagc ccagtctggg actgggaaaa cagctacatt tgccatatca
      721 attctccagc agattgaatt agatctaaag gccactcagg ctttggttct ggcacccaca
      781 cgtgaattgg ctcagcagat acaaaaggtg gttatggcat taggagacta catgggtgcc
      841 tcttgtcatg cctgcattgg gggcaccaat gtgcgtgctg aggtgcagaa gctccagatg
      901 gaagctcccc atatcatcgt gggtacccct ggccgggtgt ttgacatgct taaccggaga
      961 tacctgtccc ccaaatacat caagatgttc gtactggatg aagcagatga aatgttaagc
     1021 cgagggttca aggatcagat ctatgacata ttccagaagc tcaacagcaa cacacaggta
     1081 gttttgttgt ctgctacaat gccttctgat gtccttgagg tgaccaagaa atttatgaga
     1141 gacccttgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1201 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1261 ttcgggtggg cctttctgcg tttata
//