LOCUS       BBa_K411122             1174 bp    DNA     linear       16-Feb-2025
DEFINITION  split-RFP-A+linker+split-eIF4A-A+J13002+terminator
ACCESSION   BBa_K411122
VERSION     BBa_K411122.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..227
                     /label=TetR 1
     misc_feature    209..262
                     /label=BBa_R0040
     promoter        228..233
                     /label=-35
     misc_feature    234..252
                     /label=TetR 2
     promoter        251..256
                     /label=-10
     promoter        209..262
                     /label=BBa_R0040
     misc_feature    275..278
                     /label=conserved
     RBS             271..282
                     /label=BBa_B0034
     promoter        209..282
                     /label=BBa_J13002
     CDS             289..1092
                     /label=BBa_K411102
     misc_feature    209..1092
                     /label=BBa_K411112
     misc_feature    1101..1119
                     /label=TetR 1
     misc_feature    1101..1154
                     /label=BBa_R0040
     promoter        1120..1125
                     /label=-35
     misc_feature    1126..1144
                     /label=TetR 2
     promoter        1143..1148
                     /label=-10
     promoter        1101..1154
                     /label=BBa_R0040
     misc_feature    1167..1170
                     /label=conserved
     RBS             1163..1174
                     /label=BBa_B0034
     promoter        1101..1174
                     /label=BBa_J13002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc cctatcagtg atagagattg acatccctat
      241 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gaagaatgga
      301 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      361 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      421 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      481 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggcagc
      541 agcggcagca gcggcagcgg cagcattcgg attcttgtca agaaggaaga attgaccctg
      601 gagggtatcc gccaattcta catcaatgtg gaacgagagg agtggaagct tgacacattg
      661 tgtgacttgt atgagacgct gaccatcacc caggcagtca tctttatcaa caccagaagg
      721 aaggtggact ggctcaccga gaagatgcat gcccgagatt tcactgtttc tgccatgcac
      781 ggagatatgg accaaaagga acgagatgtg atcatgaggg agttccggtc tggctctagc
      841 agagtattaa ttaccactga cctgttggcc agaggcattg atgtgcagca ggtctcctta
      901 gtcatcaact atgaccttcc caccaacagg gaaaactaca tccacagaat cggtcgaggt
      961 ggtcggtttg gtcgtaaggg tgtggctatt aacatggtga ccgaagaaga caagaggact
     1021 cttcgagaca ttgagacttt ctacaacacc tccattgaag agatgcccct caacgttgct
     1081 gacctcattt gatactagag tccctatcag tgatagagat tgacatccct atcagtgata
     1141 gagatactga gcactactag agaaagagga gaaa
//