LOCUS       BBa_K411124              991 bp    DNA     linear       29-Jun-2024
DEFINITION  Split RFP-eIF4A_B Generator (R0040 + B0034 + RFP_split_B+Linker+eIF
            4A_split_B + B0015)
ACCESSION   BBa_K411124
VERSION     BBa_K411124.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..299
                     /label=RFP-split-B
     CDS             300..329
                     /label=linker
     misc_feature    330..851
                     /label=eIF4A-split-B
     misc_feature    852..854
                     /label=stop
     CDS             81..854
                     /label=BBa_K411104
     stem_loop       874..917
                     /label=stem_loop
     misc_feature    863..942
                     /label=BBa_B0010
     terminator      863..942
                     /label=BBa_B0010
     stem_loop       958..977
                     /label=T7 TE
     misc_feature    984..984
                     /label=stop
     polyA_site      978..991
                     /label=polya
     misc_feature    951..991
                     /label=BBa_B0012
     terminator      951..991
                     /label=BBa_B0012
     terminator      863..991
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      121 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      181 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      241 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgctggca
      301 gcagcggcag cagcggcagc ggcagcattc ggattcttgt caagaaggaa gaattgaccc
      361 tggagggtat ccgccaattc tacatcaatg tggaacgaga ggagtggaag cttgacacat
      421 tgtgtgactt gtatgagacg ctgaccatca cccaggcagt catctttatc aacaccagaa
      481 ggaaggtgga ctggctcacc gagaagatgc atgcccgaga tttcactgtt tctgccatgc
      541 acggagatat ggaccaaaag gaacgagatg tgatcatgag ggagttccgg tctggctcta
      601 gcagagtatt aattaccact gacctgttgg ccagaggcat tgatgtgcag caggtctcct
      661 tagtcatcaa ctatgacctt cccaccaaca gggaaaacta catccacaga atcggtcgag
      721 gtggtcggtt tggtcgtaag ggtgtggcta ttaacatggt gaccgaagaa gacaagagga
      781 ctcttcgaga cattgagact ttctacaaca cctccattga agagatgccc ctcaacgttg
      841 ctgacctcat ttgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      901 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      961 tcaccttcgg gtgggccttt ctgcgtttat a
//