##gff-version 3 ##sequence-region BBa_K411124 1 991 BBa_K411124 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_K411124 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K411124 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K411124 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K411124 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K411124 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_K411124 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K411124 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K411124 . CDS 81 854 . + 0 ID=BBa_K411104;Name=BBa_K411104 BBa_K411124 . sequence_feature 330 851 . + 0 ID=annotation2094531;Name=eIF4A-split-B;Parent=BBa_K411104 BBa_K411124 . CDS 300 329 . + 0 ID=annotation2094293;Name=linker;Parent=BBa_K411104 BBa_K411124 . CDS 81 299 . + 0 ID=annotation2094292;Name=RFP-split-B;Parent=BBa_K411104 BBa_K411124 . stop_codon 852 854 . + 0 ID=annotation2094532;Name=stop;Parent=BBa_K411104 BBa_K411124 . terminator 863 991 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K411124 . terminator 863 942 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K411124 . stem_loop 874 917 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K411124 . engineered_region 863 942 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K411124 . terminator 951 991 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K411124 . polyA_site 978 991 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K411124 . stem_loop 958 977 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K411124 . engineered_region 951 991 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K411124 . stop_codon 984 984 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 >BBa_K411124 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatgggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacc acctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacg aagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctggcagcagcggcagcagcgg cagcggcagcattcggattcttgtcaagaaggaagaattgaccctggagggtatccgccaattctacatcaatgtggaa cgagaggagtggaagcttgacacattgtgtgacttgtatgagacgctgaccatcacccaggcagtcatctttatcaaca ccagaaggaaggtggactggctcaccgagaagatgcatgcccgagatttcactgtttctgccatgcacggagatatgga ccaaaaggaacgagatgtgatcatgagggagttccggtctggctctagcagagtattaattaccactgacctgttggcc agaggcattgatgtgcagcaggtctccttagtcatcaactatgaccttcccaccaacagggaaaactacatccacagaa tcggtcgaggtggtcggtttggtcgtaagggtgtggctattaacatggtgaccgaagaagacaagaggactcttcgaga cattgagactttctacaacacctccattgaagagatgcccctcaacgttgctgacctcatttgatactagagccaggca tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag agtcacactggctcaccttcgggtgggcctttctgcgtttata