LOCUS       BBa_K411135              405 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac + eIF4A binding aptamer + Terminator
ACCESSION   BBa_K411135
VERSION     BBa_K411135.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             209..268
                     /label=eIF4A binding aptamer
     misc_feature    209..268
                     /label=BBa_K411105
     stem_loop       288..331
                     /label=stem_loop
     misc_feature    277..356
                     /label=BBa_B0010
     terminator      277..356
                     /label=BBa_B0010
     stem_loop       372..391
                     /label=T7 TE
     misc_feature    398..398
                     /label=stop
     polyA_site      392..405
                     /label=polya
     misc_feature    365..405
                     /label=BBa_B0012
     terminator      365..405
                     /label=BBa_B0012
     terminator      277..405
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac actcggagga cagcttagat gcaaagccgg
      241 agtgagtgta caccccgcgc caggggaata ctagagccag gcatcaaata aaacgaaagg
      301 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      361 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//