LOCUS       BBa_K411227              908 bp    DNA     linear       08-Mar-2025
DEFINITION  B0034+GFPLVA+B0015
ACCESSION   BBa_K411227
VERSION     BBa_K411227.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    733..765
                     /label=LVA tag
     misc_feature    19..732
                     /label=GFP
     misc_feature    766..771
                     /label=stop
     CDS             19..771
                     /label=BBa_K411207
     stem_loop       791..834
                     /label=stem_loop
     misc_feature    780..859
                     /label=BBa_B0010
     terminator      780..859
                     /label=BBa_B0010
     stem_loop       875..894
                     /label=T7 TE
     misc_feature    901..901
                     /label=stop
     polyA_site      895..908
                     /label=polya
     misc_feature    868..908
                     /label=BBa_B0012
     terminator      868..908
                     /label=BBa_B0012
     terminator      780..908
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aagctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
      781 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      841 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      901 cgtttata
//