LOCUS BBa_K411231 1081 bp DNA linear 22-Nov-2024 DEFINITION CFP reporter (R0010+B0034+CFP+B0015) ACCESSION BBa_K411231 VERSION BBa_K411231.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..944 /label=CFP CDS 227..944 /label=BBa_K411201 stem_loop 964..1007 /label=stem_loop misc_feature 953..1032 /label=BBa_B0010 terminator 953..1032 /label=BBa_B0010 stem_loop 1048..1067 /label=T7 TE misc_feature 1074..1074 /label=stop polyA_site 1068..1081 /label=polya misc_feature 1041..1081 /label=BBa_B0012 terminator 1041..1081 /label=BBa_B0012 terminator 953..1081 /label=BBa_B0015 misc_feature 209..1081 /label=BBa_K411221 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg 241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg 301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 421 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 661 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagatactag agccaggcat 961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1081 a //