LOCUS       BBa_K411232             1080 bp    DNA     linear       14-Jun-2024
DEFINITION  YFP reporter (R0010+B0034+YFP+B0015)
ACCESSION   BBa_K411232
VERSION     BBa_K411232.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..943
                     /label=YFP
     CDS             227..943
                     /label=BBa_K411202
     stem_loop       963..1006
                     /label=stem_loop
     misc_feature    952..1031
                     /label=BBa_B0010
     terminator      952..1031
                     /label=BBa_B0010
     stem_loop       1047..1066
                     /label=T7 TE
     misc_feature    1073..1073
                     /label=stop
     polyA_site      1067..1080
                     /label=polya
     misc_feature    1040..1080
                     /label=BBa_B0012
     terminator      1040..1080
                     /label=BBa_B0012
     terminator      952..1080
                     /label=BBa_B0015
     misc_feature    209..1080
                     /label=BBa_K411222
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      421 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      661 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
      841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtactaga gccaggcatc
      961 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1021 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//