LOCUS       BBa_K411234             1070 bp    DNA     linear       22-Dec-2024
DEFINITION  RFP Reporter (R0010+B0034+RFP+B0015)
ACCESSION   BBa_K411234
VERSION     BBa_K411234.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..902
                     /label=mrfp1
     misc_feature    909..933
                     /label=Help:Barcodes
     CDS             228..933
                     /label=BBa_E1010
     stem_loop       953..996
                     /label=stem_loop
     misc_feature    942..1021
                     /label=BBa_B0010
     terminator      942..1021
                     /label=BBa_B0010
     stem_loop       1037..1056
                     /label=T7 TE
     misc_feature    1063..1063
                     /label=stop
     polyA_site      1057..1070
                     /label=polya
     misc_feature    1030..1070
                     /label=BBa_B0012
     terminator      1030..1070
                     /label=BBa_B0012
     terminator      942..1070
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gcttcctccg
      241 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
      301 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
      361 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
      421 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
      481 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
      541 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
      601 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
      661 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
      721 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
      781 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
      841 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
      901 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
      961 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1021 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//