LOCUS       BBa_K412000              983 bp    DNA     linear       01-Jun-2024
DEFINITION  PyeaR+GFP
ACCESSION   BBa_K412000
VERSION     BBa_K412000.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     misc_feature    113..116
                     /label=conserved
     RBS             109..120
                     /label=BBa_B0034
     CDS             127..846
                     /label=GFP protein
     CDS             127..846
                     /label=BBa_E0040
     stem_loop       866..909
                     /label=stem_loop
     misc_feature    855..934
                     /label=BBa_B0010
     terminator      855..934
                     /label=BBa_B0010
     stem_loop       950..969
                     /label=T7 TE
     misc_feature    976..976
                     /label=stop
     polyA_site      970..983
                     /label=polya
     misc_feature    943..983
                     /label=BBa_B0012
     terminator      943..983
                     /label=BBa_B0012
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagaa agaggagaaa
      121 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      181 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      241 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      301 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      361 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      421 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      481 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      541 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      601 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      661 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      721 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      841 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      961 gggtgggcct ttctgcgttt ata
//