LOCUS       BBa_K412002             2060 bp    DNA     linear       25-Nov-2024
DEFINITION  LacI+RFP, PyeaR+GFP
ACCESSION   BBa_K412002
VERSION     BBa_K412002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     misc_feature    1127..1142
                     /label=NarL-binding region
     misc_feature    1135..1157
                     /label=NsrR-binding region
     misc_feature    1143..1148
                     /label=promoter -35 region
     misc_feature    1166..1171
                     /label=promoter -10 region
     promoter        1078..1177
                     /label=BBa_K216005
     misc_feature    1190..1193
                     /label=conserved
     RBS             1186..1197
                     /label=BBa_B0034
     CDS             1204..1923
                     /label=GFP protein
     CDS             1204..1923
                     /label=BBa_E0040
     stem_loop       1943..1986
                     /label=stem_loop
     misc_feature    1932..2011
                     /label=BBa_B0010
     terminator      1932..2011
                     /label=BBa_B0010
     stem_loop       2027..2046
                     /label=T7 TE
     misc_feature    2053..2053
                     /label=stop
     polyA_site      2047..2060
                     /label=polya
     misc_feature    2020..2060
                     /label=BBa_B0012
     terminator      2020..2060
                     /label=BBa_B0012
     misc_feature    1078..2060
                     /label=BBa_K412000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttc
     1081 ccatctataa tcctccctga ttcttcgctg atatggtgct aaaaagtaac caataaatgg
     1141 tatttaaaat gcaaattatc aggcgtaccc tgaaacgtac tagagaaaga ggagaaatac
     1201 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1261 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1321 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     1381 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     1441 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1501 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1561 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1621 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     1681 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     1741 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1801 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1861 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     1921 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1981 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2041 tgggcctttc tgcgtttata
//