LOCUS       BBa_K412003             1909 bp    DNA     linear       26-Dec-2024
DEFINITION  LacI+RFP, PyeaR+RFP
ACCESSION   BBa_K412003
VERSION     BBa_K412003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     misc_feature    1127..1142
                     /label=NarL-binding region
     misc_feature    1135..1157
                     /label=NsrR-binding region
     misc_feature    1143..1148
                     /label=promoter -35 region
     misc_feature    1166..1171
                     /label=promoter -10 region
     promoter        1078..1177
                     /label=BBa_K216005
     misc_feature    1190..1193
                     /label=conserved
     RBS             1186..1197
                     /label=BBa_B0034
     CDS             1204..1878
                     /label=mrfp1
     misc_feature    1885..1909
                     /label=Help:Barcodes
     CDS             1204..1909
                     /label=BBa_E1010
     misc_feature    1186..1909
                     /label=BBa_K093005
     misc_feature    1078..1909
                     /label=BBa_K412001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttc
     1081 ccatctataa tcctccctga ttcttcgctg atatggtgct aaaaagtaac caataaatgg
     1141 tatttaaaat gcaaattatc aggcgtaccc tgaaacgtac tagagaaaga ggagaaatac
     1201 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1261 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1321 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1381 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1441 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1501 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1561 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1621 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1681 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1741 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1801 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     1861 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgc
//