LOCUS       BBa_K412006             1027 bp    DNA     linear       09-Dec-2025
DEFINITION  PalkB + GFP transcriptional fusion
ACCESSION   BBa_K412006
VERSION     BBa_K412006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..143
                     /label=BBa_K412005
     RBS             152..164
                     /label=RBS-3\Weak
     RBS             158..161
                     /label=RBS
     misc_feature    152..164
                     /label=BBa_B0032
     RBS             152..164
                     /label=BBa_B0032
     CDS             171..890
                     /label=GFP protein
     CDS             171..890
                     /label=BBa_E0040
     stem_loop       910..953
                     /label=stem_loop
     misc_feature    899..978
                     /label=BBa_B0010
     terminator      899..978
                     /label=BBa_B0010
     stem_loop       994..1013
                     /label=T7 TE
     misc_feature    1020..1020
                     /label=stop
     polyA_site      1014..1027
                     /label=polya
     misc_feature    987..1027
                     /label=BBa_B0012
     terminator      987..1027
                     /label=BBa_B0012
     misc_feature    152..1027
                     /label=BBa_E0240
ORIGIN
        1 tccaattttt attaaattag tcgctacgag atttaagacg taattttatg cctaactgag
       61 aaagttaagc cgcccactct cactctcgac atcttaaacc tgagctaatc ggacgcttgc
      121 gccaactaca cctacgggta gtttactaga gtcacacagg aaagtactag atgcgtaaag
      181 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      241 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      301 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      361 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      421 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      481 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      541 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      601 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      661 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      721 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      781 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      841 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      961 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1021 gtttata
//