BBa_K414001 1 BBa_K414001 Strong Plant RBS (Kozak sequence) + GFP from E0040 2010-09-16T11:00:00Z 2015-05-08T01:12:26Z The Kozak sequence was directly engineered into the prefix primer and used to PCR out the GFP gene from E0040. In order for Green Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in GFP. false false _534_ 0 6837 9 It's complicated false We chose a strong plant Kozak sequence that can function in a broad range of dicot species, including tobacco and Arabidopsis. false Elaine Bersaba annotation2080199 1 Kpn I range2080199 1 1 6 annotation2080200 1 Kozak range2080200 1 7 18 annotation2080203 1 Translational Stop 2x range2080203 1 733 738 annotation2080201 1 Translational Start range2080201 1 19 21 annotation2080202 1 GFP ( E0040 ) range2080202 1 19 732 BBa_K414001_sequence 1 ggtaccaaaaaaaaaacaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z