BBa_K414003
1
BBa_K414003
Strong Plant RBS (Kozak sequence) + mCherry from J06504
2010-09-27T11:00:00Z
2015-05-08T01:12:26Z
The source of mCherry was derived using modified primers containing the Kozak sequence designed to bind to the iGEM part BBa_J06702 (Wang,2005)
In order for mCherry Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in mCherry.
One point mutation that should be noted is nucleotide 483 of J06504 (mCherry). C -> T is the mutation, but examining the translation of the sequence in the proper reading frame yields no translational discrepancies. Also, no illegal iGEM restriction sites were generated from the point mutation.
false
false
_534_
0
7418
9
It's complicated
false
One consideration we made was the strength of the RBS we wanted upstream of the reporter. Evidence can be found in the literature that a spectrum of translational activity can be generated by tampering with the Kozak sequence in plants. Future teams may wish to investigate how they can tamper with their Kozak of designing a system that requires discrete but relative expression of genes initiated by the same promoters.
false
Matthew Polasko
annotation2080977
1
Translational Start
range2080977
1
19
19
annotation2080979
1
Translational Stop 2x
range2080979
1
727
727
annotation2080978
1
mCherry (J06504)
range2080978
1
19
726
annotation2080976
1
Kpn I
range2080976
1
1
6
annotation2080980
1
C -> T
range2080980
1
501
501
annotation2080975
1
Kozak
range2080975
1
7
18
BBa_K414003_sequence
1
ggtaccaaaaaaaaaacaatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgctctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z