BBa_K414002 1 BBa_K414002 35S promoter 2010-09-16T11:00:00Z 2015-05-08T01:12:26Z The part was obtained from PCR of the pK7FWG2 plasmid. 35S is a plant specific promoter, obtained from Cauliflower Mosaic Virus, and the part is intended for use as a constitutive promoter for gene expression experiments. 35S works best in dicots. false false _534_ 0 6586 9 It's complicated false No special design considerations were needed in making this part. false Bryson A. Wheeler annotation2080204 1 35S promoter range2080204 1 1 1029 BBa_K414006 1 BBa_K414006 35S Promoter + (Strong Plant RBS + GFP) From K414001 2010-10-17T11:00:00Z 2015-05-08T01:12:26Z This part was made with the 35S from BBa_K414002 (Wheeler, 2010) who obtained it from pK7FWG2 plasmid. Also the GFP with strong plant RBS was taken from BBa_K414001 (Bersaba, 2010). This composite was designed to bring together a constitutive plant promoter (35S) with a fluorescent reporter (GFP) that has a strong plant RBS (Kozak)upstream to maximize translational efficiency. A composite part like this one may be used as an internal control in plant transformations to measure relative, induced expression of an inducible gene. false false _534_ 0 7418 9 It's complicated false No additional design considerations. Standard part assembly protocol followed. false Matthew Polasko component2089455 1 BBa_K414001 component2089449 1 BBa_K414002 annotation2089449 1 BBa_K414002 range2089449 1 1 1029 annotation2089455 1 BBa_K414001 range2089455 1 1038 1775 BBa_K414001 1 BBa_K414001 Strong Plant RBS (Kozak sequence) + GFP from E0040 2010-09-16T11:00:00Z 2015-05-08T01:12:26Z The Kozak sequence was directly engineered into the prefix primer and used to PCR out the GFP gene from E0040. In order for Green Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in GFP. false false _534_ 0 6837 9 It's complicated false We chose a strong plant Kozak sequence that can function in a broad range of dicot species, including tobacco and Arabidopsis. false Elaine Bersaba annotation2080201 1 Translational Start range2080201 1 19 21 annotation2080202 1 GFP ( E0040 ) range2080202 1 19 732 annotation2080203 1 Translational Stop 2x range2080203 1 733 738 annotation2080199 1 Kpn I range2080199 1 1 6 annotation2080200 1 Kozak range2080200 1 7 18 BBa_K414002_sequence 1 gactagagccaagctgatctcctttgccccggagatcaccatggacgactttctctatctctacgatctaggaagaaagttcgacggagaaggtgacgataccatgttcaccaccgataatgagaagattagcctcttcaatttcagaaagaatgctgacccacagatggttagagaggcctacgcggcaggtctcatcaagacgatctacccgagtaataatctccaggagatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcataaaccaaggcaagtaatagagattggagtctctaagaaagtagttcctactgaatcaaaggccatggagtcaaaaattcagatcgaggatctaacagaactcgccgtgaagactggcgaacagttcatacagagtcttttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcccctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggactccggtatttttacaacaattaccacaacaaaacaaacaacaaacaacattacaatttactattctagtcga BBa_K414001_sequence 1 ggtaccaaaaaaaaaacaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K414006_sequence 1 gactagagccaagctgatctcctttgccccggagatcaccatggacgactttctctatctctacgatctaggaagaaagttcgacggagaaggtgacgataccatgttcaccaccgataatgagaagattagcctcttcaatttcagaaagaatgctgacccacagatggttagagaggcctacgcggcaggtctcatcaagacgatctacccgagtaataatctccaggagatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcataaaccaaggcaagtaatagagattggagtctctaagaaagtagttcctactgaatcaaaggccatggagtcaaaaattcagatcgaggatctaacagaactcgccgtgaagactggcgaacagttcatacagagtcttttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcccctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggactccggtatttttacaacaattaccacaacaaaacaaacaacaaacaacattacaatttactattctagtcgatactagagggtaccaaaaaaaaaacaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z