LOCUS       BBa_K415000              974 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux/cI-OR : RBS-mCherry : Term
ACCESSION   BBa_K415000
VERSION     BBa_K415000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..834
                     /label=mCherry
     misc_feature    475..475
                     /label=C->T (removing PstI site)
     misc_feature    124..837
                     /label=BBa_J06504
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tata
//