LOCUS       BBa_K415002             1961 bp    DNA     linear       27-Dec-2024
DEFINITION  P_const(high) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term
ACCESSION   BBa_K415002
VERSION     BBa_K415002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_K091204
     misc_feature    988..1084
                     /label=BBa_R0065
     misc_feature    993..1012
                     /label=Lux Box
     promoter        1034..1039
                     /label=-10
     misc_feature    1044..1060
                     /label=OR2 cI
     promoter        1045..1045
                     /label=lux p(R) start
     promoter        1058..1063
                     /label=-35
     misc_feature    1068..1084
                     /label=OR1 cI
     promoter        1081..1084
                     /label=-10
     promoter        988..1084
                     /label=BBa_R0065
     misc_feature    1097..1100
                     /label=conserved
     RBS             1093..1104
                     /label=BBa_B0034
     CDS             1111..1821
                     /label=mCherry
     misc_feature    1462..1462
                     /label=C->T (removing PstI site)
     misc_feature    1111..1824
                     /label=BBa_J06504
     stem_loop       1844..1887
                     /label=stem_loop
     misc_feature    1833..1912
                     /label=BBa_B0010
     terminator      1833..1912
                     /label=BBa_B0010
     stem_loop       1928..1947
                     /label=T7 TE
     misc_feature    1954..1954
                     /label=stop
     polyA_site      1948..1961
                     /label=polya
     misc_feature    1921..1961
                     /label=BBa_B0012
     terminator      1921..1961
                     /label=BBa_B0012
     misc_feature    988..1961
                     /label=BBa_K415000
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagtaa gcacctgtag gatcgtacag gtttacgcaa
     1021 gaaaatggtt tgttatagtc gaataacacc gtgcgtgttg actattttac ctctggcggt
     1081 gatatactag agaaagagga gaaatactag atggtgagca agggcgagga ggataacatg
     1141 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     1201 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1261 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     1321 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     1381 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     1441 accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc
     1501 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1561 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     1621 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     1681 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     1741 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     1801 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
     1861 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//