##gff-version 3
##sequence-region BBa_K415002 1 1961
BBa_K415002	.	engineered_region	988	1961	.	+	0	ID=BBa_K415000;Name=BBa_K415000
BBa_K415002	.	terminator	1833	1912	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K415000
BBa_K415002	.	stem_loop	1844	1887	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415002	.	engineered_region	1833	1912	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415002	.	terminator	1921	1961	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K415000
BBa_K415002	.	stem_loop	1928	1947	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415002	.	stop_codon	1954	1954	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415002	.	polyA_site	1948	1961	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415002	.	engineered_region	1921	1961	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415002	.	engineered_region	1111	1824	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K415000
BBa_K415002	.	sequence_alteration	1462	1462	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K415002	.	CDS	1111	1821	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K415002	.	promoter	988	1084	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415000
BBa_K415002	.	non_covalent_binding_site	1068	1084	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415002	.	promoter	1058	1063	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415002	.	non_covalent_binding_site	1044	1060	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415002	.	promoter	1045	1045	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415002	.	promoter	1034	1039	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415002	.	non_covalent_binding_site	993	1012	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415002	.	promoter	1081	1084	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K415002	.	engineered_region	988	1084	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415002	.	ribosome_entry_site	1093	1104	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K415000
BBa_K415002	.	conserved_region	1097	1100	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415002	.	engineered_region	1	979	.	+	0	ID=BBa_K091204;Name=BBa_K091204
BBa_K415002	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K091204
BBa_K415002	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K415002	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415002	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415002	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K415002	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415002	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415002	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415002	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415002	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K415002	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K415002	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K415002	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K415002	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K415002	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K415002	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K415002	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K415002	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415002	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K091204
>BBa_K415002
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatg
gtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaata
ctagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggc
tccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctga
aggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgt
gaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttc
gaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcg
gcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccc
cgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaag
accacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccaca
acgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaa
gtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata