LOCUS BBa_K415007 2891 bp DNA linear 29-Nov-2024 DEFINITION pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term ACCESSION BBa_K415007 VERSION BBa_K415007.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 124..834 /label=mCherry misc_feature 475..475 /label=C->T (removing PstI site) misc_feature 124..837 /label=BBa_J06504 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 1..974 /label=BBa_K415000 misc_feature 983..1079 /label=BBa_R0065 misc_feature 988..1007 /label=Lux Box promoter 1029..1034 /label=-10 misc_feature 1039..1055 /label=OR2 cI promoter 1040..1040 /label=lux p(R) start promoter 1053..1058 /label=-35 misc_feature 1063..1079 /label=OR1 cI promoter 1076..1079 /label=-10 promoter 983..1079 /label=BBa_R0065 misc_feature 1092..1095 /label=conserved RBS 1088..1099 /label=BBa_B0034 misc_feature 1685..1716 /label=LVA CDS 1106..1684 /label=luxI misc_feature 1106..1723 /label=BBa_C0061 misc_feature 1724..1748 /label=Help:Barcodes CDS 1106..1723 /label=BBa_C0061 stem_loop 1768..1811 /label=stem_loop misc_feature 1757..1836 /label=BBa_B0010 terminator 1757..1836 /label=BBa_B0010 stem_loop 1852..1871 /label=T7 TE misc_feature 1878..1878 /label=stop polyA_site 1872..1885 /label=polya misc_feature 1845..1885 /label=BBa_B0012 terminator 1845..1885 /label=BBa_B0012 misc_feature 1894..1912 /label=TetR 1 misc_feature 1894..1947 /label=BBa_R0040 promoter 1913..1918 /label=-35 misc_feature 1919..1937 /label=TetR 2 promoter 1936..1941 /label=-10 promoter 1894..1947 /label=BBa_R0040 misc_feature 1960..1963 /label=conserved RBS 1956..1967 /label=BBa_B0034 misc_feature 1974..1975 /label=prefix misc_feature 2147..2147 /label=T misc_feature 2465..2465 /label=A CDS 1974..2723 /label=luxR misc_feature 1974..2729 /label=BBa_C0062 misc_feature 2730..2754 /label=Help:Barcodes CDS 1974..2729 /label=BBa_C0062 stem_loop 2774..2817 /label=stem_loop misc_feature 2763..2842 /label=BBa_B0010 terminator 2763..2842 /label=BBa_B0010 stem_loop 2858..2877 /label=T7 TE misc_feature 2884..2884 /label=stop polyA_site 2878..2891 /label=polya misc_feature 2851..2891 /label=BBa_B0012 terminator 2851..2891 /label=BBa_B0012 misc_feature 983..2891 /label=BBa_I15030 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag 421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag 481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac 601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agtaagcacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata acaccgtgcg tgttgactat tttacctctg gcggtgatat 1081 actagagaaa gaggagaaat actagatgac tataatgata aaaaaatcgg attttttggc 1141 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag 1201 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa 1261 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt 1321 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag 1381 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc 1441 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca 1501 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt 1561 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg 1621 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt 1681 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 1741 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1801 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1861 tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc 1921 cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac tagatgaaaa 1981 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata 2041 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac 2101 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc 2161 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag 2221 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa 2281 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta 2341 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa 2401 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc 2461 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt 2521 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata 2581 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa 2641 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa 2701 ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag 2761 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2821 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2881 ctgcgtttat a //