##gff-version 3
##sequence-region BBa_K415007 1 2891
BBa_K415007	.	engineered_region	983	2891	.	+	0	ID=BBa_I15030;Name=BBa_I15030
BBa_K415007	.	terminator	2851	2891	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I15030
BBa_K415007	.	polyA_site	2878	2891	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415007	.	stem_loop	2858	2877	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415007	.	stop_codon	2884	2884	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415007	.	engineered_region	2851	2891	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415007	.	terminator	1845	1885	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I15030
BBa_K415007	.	polyA_site	1872	1885	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415007	.	stem_loop	1852	1871	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415007	.	stop_codon	1878	1878	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415007	.	engineered_region	1845	1885	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415007	.	terminator	2763	2842	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I15030
BBa_K415007	.	stem_loop	2774	2817	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415007	.	engineered_region	2763	2842	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415007	.	CDS	1106	1723	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_I15030
BBa_K415007	.	CDS	1106	1684	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K415007	.	engineered_region	1106	1723	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K415007	.	engineered_tag	1724	1748	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K415007	.	tag	1685	1716	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K415007	.	ribosome_entry_site	1088	1099	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I15030
BBa_K415007	.	conserved_region	1092	1095	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415007	.	ribosome_entry_site	1956	1967	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I15030
BBa_K415007	.	conserved_region	1960	1963	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415007	.	CDS	1974	2729	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I15030
BBa_K415007	.	CDS	1974	2723	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K415007	.	point_mutation	2465	2465	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K415007	.	point_mutation	2147	2147	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K415007	.	engineered_tag	2730	2754	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K415007	.	engineered_region	1974	1975	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K415007	.	engineered_region	1974	2729	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K415007	.	terminator	1757	1836	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I15030
BBa_K415007	.	stem_loop	1768	1811	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415007	.	engineered_region	1757	1836	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415007	.	promoter	983	1079	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_I15030
BBa_K415007	.	promoter	1053	1058	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415007	.	promoter	1040	1040	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415007	.	promoter	1029	1034	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	1039	1055	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	1063	1079	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415007	.	engineered_region	983	1079	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415007	.	promoter	1076	1079	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K415007	.	promoter	1894	1947	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I15030
BBa_K415007	.	non_covalent_binding_site	1894	1912	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K415007	.	non_covalent_binding_site	1919	1937	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K415007	.	promoter	1913	1918	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K415007	.	engineered_region	1894	1947	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K415007	.	promoter	1936	1941	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K415007	.	engineered_region	1	974	.	+	0	ID=BBa_K415000;Name=BBa_K415000
BBa_K415007	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K415000
BBa_K415007	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415007	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415007	.	engineered_region	124	837	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K415000
BBa_K415007	.	CDS	124	834	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K415007	.	sequence_alteration	475	475	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K415007	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K415000
BBa_K415007	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415007	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415007	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415007	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415007	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K415000
BBa_K415007	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415007	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415000
BBa_K415007	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415007	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415007	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415007	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415007	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415007	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
>BBa_K415007
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccat
catcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggc
gagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggaca
tcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtc
cttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcc
ttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaaga
agaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggct
gaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggc
gcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccg
agggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcc
ctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata