LOCUS       BBa_K415008             2026 bp    DNA     linear       29-Nov-2024
DEFINITION  pLux/cI-OR : RBS-mCherry : Term : p(lac) IQ : luxR : Term : pLux
ACCESSION   BBa_K415008
VERSION     BBa_K415008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..834
                     /label=mCherry
     misc_feature    475..475
                     /label=C->T (removing PstI site)
     misc_feature    124..837
                     /label=BBa_J06504
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     misc_feature    1..974
                     /label=BBa_K415000
     misc_feature    983..1019
                     /label=P(Lac) IQ
     promoter        985..990
                     /label=-35
     promoter        1008..1013
                     /label=-10
     promoter        983..1019
                     /label=BBa_I14032
     misc_feature    1032..1035
                     /label=conserved
     RBS             1028..1039
                     /label=BBa_B0034
     misc_feature    1046..1047
                     /label=prefix
     misc_feature    1219..1219
                     /label=T
     misc_feature    1537..1537
                     /label=A
     CDS             1046..1795
                     /label=luxR
     misc_feature    1046..1801
                     /label=BBa_C0062
     misc_feature    1802..1826
                     /label=Help:Barcodes
     CDS             1046..1801
                     /label=BBa_C0062
     stem_loop       1846..1889
                     /label=stem_loop
     misc_feature    1835..1914
                     /label=BBa_B0010
     terminator      1835..1914
                     /label=BBa_B0010
     stem_loop       1930..1949
                     /label=T7 TE
     misc_feature    1956..1956
                     /label=stop
     polyA_site      1950..1963
                     /label=polya
     misc_feature    1923..1963
                     /label=BBa_B0012
     terminator      1923..1963
                     /label=BBa_B0012
     misc_feature    1028..1963
                     /label=BBa_I0462
     misc_feature    1972..1991
                     /label=LuxR/HSL
     promoter        1991..1996
                     /label=-35
     promoter        2013..2018
                     /label=-10
     misc_feature    2024..2024
                     /label=start
     misc_feature    1972..2026
                     /label=BBa_R0062
     promoter        1972..2026
                     /label=BBa_R0062
     misc_feature    983..2026
                     /label=BBa_J09855
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tatatactag agtggtgcaa aacctttcgc ggtatggcat gatagcgcct
     1021 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat
     1081 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat
     1141 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt
     1201 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga
     1261 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat
     1321 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga
     1381 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg
     1441 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt
     1501 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat
     1561 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc
     1621 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg
     1681 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca
     1741 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata
     1801 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
     1861 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1921 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg
     1981 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaa
//