##gff-version 3
##sequence-region BBa_K415008 1 2026
BBa_K415008	.	engineered_region	1	974	.	+	0	ID=BBa_K415000;Name=BBa_K415000
BBa_K415008	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K415000
BBa_K415008	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415008	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415008	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K415000
BBa_K415008	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415008	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415008	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415008	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415008	.	engineered_region	124	837	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K415000
BBa_K415008	.	CDS	124	834	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K415008	.	sequence_alteration	475	475	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K415008	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415000
BBa_K415008	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415008	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415008	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415008	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415008	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K415008	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415008	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415008	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415008	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K415000
BBa_K415008	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415008	.	engineered_region	983	2026	.	+	0	ID=BBa_J09855;Name=BBa_J09855
BBa_K415008	.	promoter	983	1019	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K415008	.	operator	983	1019	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K415008	.	promoter	1008	1013	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K415008	.	promoter	985	990	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K415008	.	promoter	1972	2026	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K415008	.	promoter	1991	1996	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K415008	.	non_covalent_binding_site	1972	1991	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K415008	.	promoter	2013	2018	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K415008	.	engineered_region	1972	2026	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K415008	.	start_codon	2024	2024	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K415008	.	engineered_region	1028	1963	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K415008	.	terminator	1835	1914	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K415008	.	engineered_region	1835	1914	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415008	.	stem_loop	1846	1889	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415008	.	terminator	1923	1963	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K415008	.	stem_loop	1930	1949	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415008	.	stop_codon	1956	1956	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415008	.	engineered_region	1923	1963	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415008	.	polyA_site	1950	1963	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415008	.	CDS	1046	1801	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K415008	.	engineered_region	1046	1047	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K415008	.	engineered_tag	1802	1826	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K415008	.	point_mutation	1537	1537	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K415008	.	CDS	1046	1795	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K415008	.	engineered_region	1046	1801	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K415008	.	point_mutation	1219	1219	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K415008	.	ribosome_entry_site	1028	1039	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K415008	.	conserved_region	1032	1035	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K415008
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccat
catcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggc
gagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggaca
tcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtc
cttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcc
ttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaaga
agaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggct
gaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggc
gcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccg
agggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagag
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacct
gtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa