LOCUS       BBa_K415009             2043 bp    DNA     linear       21-Dec-2024
DEFINITION  pLux/cI-OR : RBS-mCherry : Term : P(tetR) : RBS-luxR : Term : pLux
ACCESSION   BBa_K415009
VERSION     BBa_K415009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..834
                     /label=mCherry
     misc_feature    475..475
                     /label=C->T (removing PstI site)
     misc_feature    124..837
                     /label=BBa_J06504
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     misc_feature    1..974
                     /label=BBa_K415000
     misc_feature    983..1001
                     /label=TetR 1
     misc_feature    983..1036
                     /label=BBa_R0040
     promoter        1002..1007
                     /label=-35
     misc_feature    1008..1026
                     /label=TetR 2
     promoter        1025..1030
                     /label=-10
     promoter        983..1036
                     /label=BBa_R0040
     misc_feature    1049..1052
                     /label=conserved
     RBS             1045..1056
                     /label=BBa_B0034
     misc_feature    1063..1064
                     /label=prefix
     misc_feature    1236..1236
                     /label=T
     misc_feature    1554..1554
                     /label=A
     CDS             1063..1812
                     /label=luxR
     misc_feature    1063..1818
                     /label=BBa_C0062
     misc_feature    1819..1843
                     /label=Help:Barcodes
     CDS             1063..1818
                     /label=BBa_C0062
     stem_loop       1863..1906
                     /label=stem_loop
     misc_feature    1852..1931
                     /label=BBa_B0010
     terminator      1852..1931
                     /label=BBa_B0010
     stem_loop       1947..1966
                     /label=T7 TE
     misc_feature    1973..1973
                     /label=stop
     polyA_site      1967..1980
                     /label=polya
     misc_feature    1940..1980
                     /label=BBa_B0012
     terminator      1940..1980
                     /label=BBa_B0012
     terminator      1852..1980
                     /label=BBa_B0015
     misc_feature    1989..2008
                     /label=LuxR/HSL
     promoter        2008..2013
                     /label=-35
     promoter        2030..2035
                     /label=-10
     misc_feature    2041..2041
                     /label=start
     misc_feature    1989..2043
                     /label=BBa_R0062
     promoter        1989..2043
                     /label=BBa_R0062
     misc_feature    983..2043
                     /label=BBa_F2620
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga
     1021 tagagatact gagcactact agagaaagag gagaaatact agatgaaaaa cataaatgcc
     1081 gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat
     1141 caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt
     1201 tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg
     1261 aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac
     1321 tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct
     1381 ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt
     1441 catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat
     1501 atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt
     1561 gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga
     1621 gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata
     1681 ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat
     1741 acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca
     1801 tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
     1861 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1921 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     1981 tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat
     2041 aaa
//