##gff-version 3
##sequence-region BBa_K415023 1 3722
BBa_K415023	.	engineered_region	969	3722	.	+	0	ID=BBa_K415022;Name=BBa_K415022
BBa_K415023	.	engineered_region	969	2948	.	+	0	ID=BBa_K415006;Name=BBa_K415006;Parent=BBa_K415022
BBa_K415023	.	engineered_region	1951	2948	.	+	0	ID=BBa_S03119;Name=BBa_S03119;Parent=BBa_K415006
BBa_K415023	.	terminator	2908	2948	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K415023	.	stem_loop	2915	2934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415023	.	stop_codon	2941	2941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415023	.	engineered_region	2908	2948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415023	.	polyA_site	2935	2948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415023	.	CDS	2031	2786	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K415023	.	CDS	2031	2780	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K415023	.	point_mutation	2522	2522	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K415023	.	point_mutation	2204	2204	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K415023	.	engineered_region	2031	2032	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K415023	.	engineered_region	2031	2786	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K415023	.	engineered_tag	2787	2811	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K415023	.	terminator	2820	2899	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K415023	.	stem_loop	2831	2874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415023	.	engineered_region	2820	2899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415023	.	promoter	1951	2004	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K415023	.	engineered_region	1951	2004	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K415023	.	promoter	1970	1975	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K415023	.	non_covalent_binding_site	1976	1994	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K415023	.	promoter	1993	1998	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K415023	.	non_covalent_binding_site	1951	1969	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K415023	.	ribosome_entry_site	2013	2024	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K415023	.	conserved_region	2017	2020	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415023	.	engineered_region	969	1942	.	+	0	ID=BBa_K415000;Name=BBa_K415000;Parent=BBa_K415006
BBa_K415023	.	terminator	1814	1893	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K415000
BBa_K415023	.	stem_loop	1825	1868	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415023	.	engineered_region	1814	1893	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415023	.	engineered_region	1092	1805	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K415000
BBa_K415023	.	sequence_alteration	1443	1443	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K415023	.	CDS	1092	1802	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K415023	.	ribosome_entry_site	1074	1085	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K415000
BBa_K415023	.	conserved_region	1078	1081	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415023	.	terminator	1902	1942	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K415000
BBa_K415023	.	stem_loop	1909	1928	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415023	.	stop_codon	1935	1935	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415023	.	engineered_region	1902	1942	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415023	.	polyA_site	1929	1942	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K415023	.	promoter	969	1065	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415000
BBa_K415023	.	non_covalent_binding_site	1049	1065	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415023	.	promoter	1039	1044	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415023	.	non_covalent_binding_site	1025	1041	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415023	.	engineered_region	969	1065	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415023	.	promoter	1062	1065	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K415023	.	promoter	1026	1026	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415023	.	non_covalent_binding_site	974	993	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415023	.	promoter	1015	1020	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415023	.	engineered_region	2957	3722	.	+	0	ID=BBa_K415019;Name=BBa_K415019;Parent=BBa_K415022
BBa_K415023	.	promoter	2957	3053	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K415019
BBa_K415023	.	non_covalent_binding_site	3037	3053	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K415023	.	promoter	3027	3032	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K415023	.	non_covalent_binding_site	3013	3029	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K415023	.	engineered_region	2957	3053	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K415023	.	promoter	3050	3053	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K415023	.	promoter	3014	3014	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K415023	.	non_covalent_binding_site	2962	2981	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K415023	.	promoter	3003	3008	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K415023	.	engineered_region	3062	3722	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K415019
BBa_K415023	.	CDS	3080	3697	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K415023	.	CDS	3080	3658	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K415023	.	engineered_tag	3698	3722	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K415023	.	engineered_region	3080	3697	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K415023	.	tag	3659	3690	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K415023	.	ribosome_entry_site	3062	3073	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K415023	.	conserved_region	3066	3069	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K415023	.	engineered_region	1	960	.	+	0	ID=BBa_K182101;Name=BBa_K182101
BBa_K415023	.	promoter	1	74	.	+	0	ID=BBa_I751502;Name=BBa_I751502;Parent=BBa_K182101
BBa_K415023	.	sequence_feature	42	48	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_K415023	.	sequence_feature	20	26	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_K415023	.	promoter	20	74	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_K415023	.	non_covalent_binding_site	27	41	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_K415023	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_K415023	.	non_covalent_binding_site	49	74	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_K415023	.	terminator	832	911	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K182101
BBa_K415023	.	stem_loop	843	886	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K415023	.	engineered_region	832	911	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K415023	.	ribosome_entry_site	83	97	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K182101
BBa_K415023	.	ribosome_entry_site	90	94	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K415023	.	engineered_region	83	97	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K415023	.	ribosome_entry_site	83	97	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K415023	.	CDS	104	823	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K182101
BBa_K415023	.	CDS	104	823	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K415023	.	terminator	920	960	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K182101
BBa_K415023	.	stem_loop	927	946	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K415023	.	stop_codon	953	953	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K415023	.	engineered_region	920	960	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K415023	.	polyA_site	947	960	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K415023
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttacta
gagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataa
caccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggc
gaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagt
tcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccc
cctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatc
cccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtga
ccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccga
cggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaag
ggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaaga
agcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgt
ggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgac
acatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaa
tggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataa
ttaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactcc
aatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcga
aaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcaca
ttcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagtt
gataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgt
gggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaac
caatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgc
ccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgt
tatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagat
gactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaa
gtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcag
aatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgct
gaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgct
gtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacg
ctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcc
ttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaa
cagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagt
gtagatctc