BBa_K415031 1 pLux/cI-OR Improved PLux/cI-OR Promoter 2010-10-21T11:00:00Z 2015-05-08T01:12:26Z This is a hybrid promoter synthesized from a portion of the Vibrio Fischerii Lux operon and the cI/cro switch of Lambda bacteriophage. This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. false false _525_ 0 6719 9 It's complicated false This promoter was designed entirely as a result of issues with the R0065 promoter, which was essentially designed as a literal fusion of two promoters that happened to contain twin -10 transcription initiation sites. This resulted in a promoter that was not leaky without the presence of AHL, but constitutively on. This newly designed promoter should properly follow the logic detailed above. false Grant Robinson BBa_K415031_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z