LOCUS       BBa_K415049             2426 bp    DNA     linear       21-Dec-2024
DEFINITION  Cm Backbone with pBR322 Derived Origin
ACCESSION   BBa_K415049
VERSION     BBa_K415049.1  
FEATURES             Location/Qualifiers
     misc_feature    2405..2426
                     /label=Biobrick Prefix
     misc_feature    1..21
                     /label=Biobrick Suffix
     promoter        1479..1498
                     /label=Extra VR site
     promoter        116..135
                     /label=VR Site
     promoter        2281..2301
                     /label=VF2 Site
     misc_feature    184..952
                     /label=P1004 (Cm resistance)
     misc_feature    1583..2197
                     /label=pMB1 Origin
     misc_feature    979..1172
                     /label=ROP
     RBS             969..974
                     /label=RBS
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gaggttgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
      241 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      301 aatcacggga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      361 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      421 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      481 ccgcctgatg aacgctcacc cggagtttcg tatggccatg aaagacggtg agctggtgat
      541 ctgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcgtc
      601 cctctggagt gaataccacg acgatttccg gcagtttctc cacatatatt cgcaagatgt
      661 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      721 tgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      781 caacttcttc gcccccgttt tcacgatggg caaatattat acgcaaggcg acaaggtgct
      841 gatgccgctg gcgatccagg ttcatcatgc cgtttgtgat ggcttccatg tcggccgcat
      901 gcttaatgaa ttacaacagt actgtgatga gtggcagggc ggggcgtaat aatactagag
      961 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     1021 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     1081 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     1141 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1201 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1261 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     1321 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     1381 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     1441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttactagct ccggcaaaaa
     2221 aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
     2281 ttgccacctg acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc
     2341 cacccgtgaa ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg
     2401 actcgaattc gcggccgctt ctagag
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