LOCUS BBa_K415069 3693 bp DNA linear 26-Nov-2024 DEFINITION pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fi xed-pLux/cI:LuxI ACCESSION BBa_K415069 VERSION BBa_K415069.1 FEATURES Location/Qualifiers promoter 20..74 /label=plac misc_feature 20..26 /label=-35 misc_feature 42..48 /label=-10 misc_feature 27..41 /label=O1 misc_feature 49..74 /label=O1 misc_feature 1..19 /label=luxR binding site promoter 1..74 /label=BBa_I751502 RBS 83..97 /label=RBS-1\Strong RBS 90..94 /label=RBS misc_feature 83..97 /label=BBa_B0030 RBS 83..97 /label=BBa_B0030 CDS 104..823 /label=GFP protein CDS 104..823 /label=BBa_E0040 stem_loop 843..886 /label=stem_loop misc_feature 832..911 /label=BBa_B0010 terminator 832..911 /label=BBa_B0010 stem_loop 927..946 /label=T7 TE misc_feature 953..953 /label=stop polyA_site 947..960 /label=polya misc_feature 920..960 /label=BBa_B0012 terminator 920..960 /label=BBa_B0012 misc_feature 1..960 /label=BBa_K182101 misc_feature 969..1065 /label=BBa_R0065 misc_feature 974..993 /label=Lux Box promoter 1015..1020 /label=-10 misc_feature 1025..1041 /label=OR2 cI promoter 1026..1026 /label=lux p(R) start promoter 1039..1044 /label=-35 misc_feature 1049..1065 /label=OR1 cI promoter 1062..1065 /label=-10 promoter 969..1065 /label=BBa_R0065 misc_feature 1078..1081 /label=conserved RBS 1074..1085 /label=BBa_B0034 CDS 1092..1802 /label=mCherry misc_feature 1443..1443 /label=C->T (removing PstI site) misc_feature 1092..1805 /label=BBa_J06504 stem_loop 1825..1868 /label=stem_loop misc_feature 1814..1893 /label=BBa_B0010 terminator 1814..1893 /label=BBa_B0010 stem_loop 1909..1928 /label=T7 TE misc_feature 1935..1935 /label=stop polyA_site 1929..1942 /label=polya misc_feature 1902..1942 /label=BBa_B0012 terminator 1902..1942 /label=BBa_B0012 misc_feature 969..1942 /label=BBa_K415000 misc_feature 1951..1969 /label=TetR 1 misc_feature 1951..2004 /label=BBa_R0040 promoter 1970..1975 /label=-35 misc_feature 1976..1994 /label=TetR 2 promoter 1993..1998 /label=-10 promoter 1951..2004 /label=BBa_R0040 misc_feature 2017..2020 /label=conserved RBS 2013..2024 /label=BBa_B0034 misc_feature 2031..2032 /label=prefix misc_feature 2204..2204 /label=T misc_feature 2522..2522 /label=A CDS 2031..2780 /label=luxR misc_feature 2031..2786 /label=BBa_C0062 misc_feature 2787..2811 /label=Help:Barcodes CDS 2031..2786 /label=BBa_C0062 stem_loop 2831..2874 /label=stem_loop misc_feature 2820..2899 /label=BBa_B0010 terminator 2820..2899 /label=BBa_B0010 stem_loop 2915..2934 /label=T7 TE misc_feature 2941..2941 /label=stop polyA_site 2935..2948 /label=polya misc_feature 2908..2948 /label=BBa_B0012 terminator 2908..2948 /label=BBa_B0012 misc_feature 1951..2948 /label=BBa_S03119 misc_feature 969..2948 /label=BBa_K415006 misc_feature 1..2948 /label=BBa_K415021 promoter 2957..3024 /label=BBa_K415031 misc_feature 3037..3040 /label=conserved RBS 3033..3044 /label=BBa_B0034 misc_feature 3630..3661 /label=LVA CDS 3051..3629 /label=luxI misc_feature 3051..3668 /label=BBa_C0061 misc_feature 3669..3693 /label=Help:Barcodes CDS 3051..3668 /label=BBa_C0061 misc_feature 3033..3693 /label=BBa_C0261 misc_feature 2957..3693 /label=BBa_K415033 ORIGIN 1 acctgtagga tcgtacaggt ttacttgtga gcggataaca atatagtgtg tggaattgtg 61 agcggataac aatttactag agattaaaga ggagaaatac tagatgcgta aaggagaaga 121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 181 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 301 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 361 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 421 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 481 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 541 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 601 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 721 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 781 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc 841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 961 tactagagta agcacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 1021 cgaataacac cgtgcgtgtt gactatttta cctctggcgg tgatatacta gagaaagagg 1081 agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 1141 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 1201 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 1261 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 1321 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 1381 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 1441 ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1501 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 1561 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 1621 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1681 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 1741 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 1801 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1921 ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct 1981 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgaaaaaca 2041 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg 2101 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg 2161 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta 2221 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt 2281 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata 2341 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt 2401 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag 2461 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt 2521 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa 2581 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt 2641 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga 2701 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg 2761 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc 2821 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2881 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2941 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 3001 agtcgaatac ctctggcggt tatatactag agaaagagga gaaatactag atgactataa 3061 tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc 3121 ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg 3181 aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa 3241 atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt 3301 ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc 3361 gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga 3421 aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag 3481 taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg 3541 gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta 3601 atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac gctttagtag 3661 cttaataact ctgatagtgc tagtgtagat ctc //