BBa_K415100
1
BBa_K415100
RBS : pVIII
2010-06-25T11:00:00Z
2015-05-08T01:12:27Z
This ~300 bp part was produced via PCR from the genome of bacteriophage M13X07. The primers read as follows:
Forward: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaaagaggagaaatactagATGAAAAAGTCTTTAGTCCTCAAAGCCTC
Reverse: [To be specified]
These primers ensured that the PCR included the standard BioBrick restriction sites.
A copy of pVIII, a surface protein that is highly expressed by bacteriophage M13. This part has applications in phage display.
false
false
_525_
0
6719
9
In stock
false
This sequence was produced via PCR, and it was determined after a graded PCR experiment that the optimal ligation temperature for pVIII was 60 degrees Celsius.
false
Grant Robinson
annotation2072815
1
pVIII
range2072815
1
19
240
annotation2072814
1
B0034 Strong RBS
range2072814
1
1
12
BBa_K415109
1
BBa_K415109
PLuxR/cI-OR : RBS : pVIII
2010-07-03T11:00:00Z
2015-05-08T01:12:27Z
The promoter portion is part BBa_R0065; the ribosome binding site and gene portion is part BBa_K415100.
This is a composite part including a copy of the surface protein pIII downstream of a promoter repressed by cI and activated by the LuxR-3OC6HSL complex; this part therefore requires either LuxI or external 3OC6HSL to function.
This part has applications in inducible phage display.
false
false
_525_
0
6719
9
Not in stock
false
There are no specific design considerations to be noted.
false
Grant Robinson
component2218814
1
BBa_K415100
component2218804
1
BBa_R0065
annotation2218814
1
BBa_K415100
range2218814
1
106
345
annotation2218804
1
BBa_R0065
range2218804
1
1
97
BBa_R0065
1
cI+luxR
Promoter (lambda cI and luxR regulated -- hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
Released HQ 2013
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P> </P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P> </P>
false
false
_1_
0
24
7
In stock
false
<P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1986776
1
-10
range1986776
1
47
52
annotation1986775
1
Lux Box
range1986775
1
6
25
annotation1986777
1
OR2 cI
range1986777
1
57
73
annotation1986774
1
BBa_R0065
range1986774
1
1
97
annotation1986781
1
-10
range1986781
1
94
97
annotation1986780
1
OR1 cI
range1986780
1
81
97
annotation1986779
1
-35
range1986779
1
71
76
annotation1986778
1
lux p(R) start
range1986778
1
58
58
BBa_K415100_sequence
1
aaagaggagaaatactagatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
BBa_K415109_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctga
BBa_R0065_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z