BBa_K415118 1 BBa_K415118 RBS Weak : pVIII-Jun 2010-07-10T11:00:00Z 2015-05-08T01:12:27Z The source of the weak RBS is part BBa_B0031. The source of the pVIII protein is the genomic sequence of the M13KE bacteriophage, and the Fos protein can be found in the PubMed nucleotide archives. A modified copy of pVIII, a surface protein that is highly expressed by bacteriophage M13. This particular copy includes a fusion with the Jun protein. This part has applications in phage display, especially when paired with the Fos protein, with which it can form a very stable heterodimer. false false _525_ 0 6719 9 Not in stock false The sequence for Jun is extensive at 1005 bp; therefore, designing the sequence as a simple primer is prohibitively costly. We instead constructed the sequence using PCR SOEing; in between the parts is a [pentaglycine linker bridge (GGAGGAGGAGGAGGA) or glycine-serine bridge (GGAGGAGGAGGAAGU).] false Grant Robinson annotation2073120 1 Jun (Murine) range2073120 1 258 1262 annotation2073117 1 B0031 RBS Weak range2073117 1 1 14 annotation2073118 1 pVIII (M13KE) range2073118 1 21 242 annotation2073119 1 Glycine-Serine Linker range2073119 1 243 257 BBa_K415118_sequence 1 tcacacaggaaacctactagatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgaggaggaggaggaagtatgactgcaaagatggaaacgaccttctacgacgatgccctcaacgcctcgttcctccagtccgagagcggtgcctacggctacagtaaccctaagatcctaaaacagagcatgaccttgaacctggccgacccggtgggcagtctgaagccgcacctccgcgccaagaactcggaccttctcacgtcgcccgacgtcgggctgctcaagctggcgtcgccggagctggagcgcctgatcatccagtccagcaatgggcacatcaccactacaccgacccccacccagttcttgtgccccaagaacgtgaccgacgagcaggagggcttcgccgagggcttcgtgcgcgccctggctgaactgcatagccagaacacgcttcccagtgtcacctccgcggcacagccggtcagcggggcgggcatggtggctcccgcggtggcctcagtagcaggcgctggcggcggtggtggctacagcgccagcctgcacagtgagcctccggtctacgccaacctcagcaacttcaacccgggtgcgctgagcagcggcggtggggcgccctcctatggcgcggccgggctggcctttccctcgcagccgcagcagcagcagcagccgcctcagccgccgcaccacttgccccaacagatcccggtgcagcacccgcggctgcaagccctgaaggaagagccgcagaccgtgccggagatgccgggagagacgccgcccctgtcccctatcgacatggagtctcaggagcggatcaaggcagagaggaagcgcatgaggaaccgcattgccgcctccaagtgccggaaaaggaagctggagcggatcgctcggctagaggaaaaagtgaaaaccttgaaagcgcaaaactccgagctggcatccacggccaacatgctcagggaacaggtggcacagcttaagcagaaagtcatgaaccacgttaacagtgggtgccaactcatgctaacgcagcagttgcaaacgttttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z