BBa_K415137 1 BBa_K415137 RBS Weak : pIII 2010-07-08T11:00:00Z 2015-05-08T01:12:27Z The RBS component is BBa_B0031, a previous BioBrick part; the copy of pIII comes from the M13KE bacteriophage strain. H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009 (Used to gather more information about the potential usefulness of our RBS selection.) This is a copy of the terminal M13 bacteriophage minor coat protein pIII placed downstream of B0031, a weak RBS. This translational unit has applications in phage display and appears to use an RBS that is significantly weaker than that used by wild type M13, according to the Voigt Lab Ribosome Binding Site Calculator. false false _525_ 0 6719 9 Not in stock false We decided to select a weaker RBS than is found in wild type in order to experiment with the effect of RBSs upon phage display and overall system dynamics. false Grant Robinson annotation2073109 1 pIII range2073109 1 23 1297 annotation2073108 1 B0031 RBS Weak range2073108 1 1 14 BBa_K415137_sequence 1 tcacacaggaaacctactagaggtgaaaaaattattattcgcaattcctttagtggtacctttctattctcactcggccgaaactgttgaaagttgtttagcaaaatcccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggctgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatttatttgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttggcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z