LOCUS       BBa_K415138             1503 bp    DNA     linear       23-Dec-2024
DEFINITION  pLac : RBS : M13 PIII
ACCESSION   BBa_K415138
VERSION     BBa_K415138.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=B0034 Strong RBS
     CDS             229..1503
                     /label=pIII
     misc_feature    209..1503
                     /label=BBa_K415108
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaggt gaaaaaatta
      241 ttattcgcaa ttcctttagt ggtacctttc tattctcact cggccgaaac tgttgaaagt
      301 tgtttagcaa aatcccatac agaaaattca tttactaacg tctggaaaga cgacaaaact
      361 ttagatcgtt acgctaacta tgagggctgt ctgtggaatg ctacaggcgt tgtagtttgt
      421 actggtgacg aaactcagtg ttacggtaca tgggttccta ttgggcttgc tatccctgaa
      481 aatgagggtg gtggctctga gggtggcggt tctgagggtg gcggttctga gggtggcggt
      541 actaaacctc ctgagtacgg tgatacacct attccgggct atacttatat caaccctctc
      601 gacggcactt atccgcctgg tactgagcaa aaccccgcta atcctaatcc ttctcttgag
      661 gagtctcagc ctcttaatac tttcatgttt cagaataata ggttccgaaa taggcagggg
      721 gcattaactg tttatacggg cactgttact caaggcactg accccgttaa aacttattac
      781 cagtacactc ctgtatcatc aaaagccatg tatgacgctt actggaacgg taaattcaga
      841 gactgcgctt tccattctgg ctttaatgag gatttatttg tttgtgaata tcaaggccaa
      901 tcgtctgacc tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg tggttctggt
      961 ggcggctctg agggtggtgg ctctgagggt ggcggttctg agggtggcgg ctctgaggga
     1021 ggcggttccg gtggtggctc tggttccggt gattttgatt atgaaaagat ggcaaacgct
     1081 aataaggggg ctatgaccga aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc
     1141 aaacttgatt ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt
     1201 tccggccttg ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg
     1261 gctcaagtcg gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct
     1321 tccctccctc aatcggttga atgtcgccct tttgtctttg gcgctggtaa accatatgaa
     1381 ttttctattg attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat
     1441 gttgccacct ttatgtatgt attttctacg tttgctaaca tactgcgtaa taaggagtct
     1501 taa
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