LOCUS BBa_K415150 2507 bp DNA linear 22-Nov-2024 DEFINITION K415010 + RBS + p8-BASE fusion ACCESSION BBa_K415150 VERSION BBa_K415150.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 124..834 /label=mCherry misc_feature 475..475 /label=C->T (removing PstI site) misc_feature 124..837 /label=BBa_J06504 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 1..974 /label=BBa_K415000 misc_feature 983..1001 /label=TetR 1 misc_feature 983..1036 /label=BBa_R0040 promoter 1002..1007 /label=-35 misc_feature 1008..1026 /label=TetR 2 promoter 1025..1030 /label=-10 promoter 983..1036 /label=BBa_R0040 misc_feature 1049..1052 /label=conserved RBS 1045..1056 /label=BBa_B0034 misc_feature 1063..1064 /label=prefix misc_feature 1236..1236 /label=T misc_feature 1554..1554 /label=A CDS 1063..1812 /label=luxR misc_feature 1063..1818 /label=BBa_C0062 misc_feature 1819..1843 /label=Help:Barcodes CDS 1063..1818 /label=BBa_C0062 stem_loop 1863..1906 /label=stem_loop misc_feature 1852..1931 /label=BBa_B0010 terminator 1852..1931 /label=BBa_B0010 stem_loop 1947..1966 /label=T7 TE misc_feature 1973..1973 /label=stop polyA_site 1967..1980 /label=polya misc_feature 1940..1980 /label=BBa_B0012 terminator 1940..1980 /label=BBa_B0012 misc_feature 983..1980 /label=BBa_S03119 misc_feature 1..1980 /label=BBa_K415006 misc_feature 1989..2085 /label=BBa_R0065 misc_feature 1994..2013 /label=Lux Box promoter 2035..2040 /label=-10 misc_feature 2045..2061 /label=OR2 cI promoter 2046..2046 /label=lux p(R) start promoter 2059..2064 /label=-35 misc_feature 2069..2085 /label=OR1 cI promoter 2082..2085 /label=-10 promoter 1989..2085 /label=BBa_R0065 misc_feature 1..2085 /label=BBa_K415010 misc_feature 2098..2101 /label=conserved RBS 2094..2105 /label=BBa_B0034 CDS 2112..2507 /label=BBa_K415123 misc_feature 2094..2507 /label=BBa_K415144 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag 421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag 481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac 601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga 1021 tagagatact gagcactact agagaaagag gagaaatact agatgaaaaa cataaatgcc 1081 gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat 1141 caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt 1201 tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg 1261 aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac 1321 tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct 1381 ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt 1441 catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat 1501 atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt 1561 gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga 1621 gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata 1681 ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat 1741 acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca 1801 tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 1861 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1921 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1981 tactagagta agcacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 2041 cgaataacac cgtgcgtgtt gactatttta cctctggcgg tgatatacta gagaaagagg 2101 agaaatacta gatgaaaaag tctttagtcc tcaaagcttc tgtagccgtt gctaccctcg 2161 ttccgatgct gtctttcgct gctcagctga aaaaaaaact gcaagctaac aaaaaaaaac 2221 tggctcagct gaaatggaaa ctgcaagctc tgaaaaaaaa actggctcag gcagccgcgg 2281 aacaaaaact catctcagaa gaggatctgc gcgccgacca cggcagccgc gccagccacg 2341 acgccagccg gcgcgctgag ggtgacgatc ccgcaaaagc ggcctacgag actatcaaag 2401 acgacatcgt taaatatatc ggttatgcgt gggcgatggt tgttgtcatt gtcggcgcaa 2461 ctatcggtat caagctgttt aagaaattca cctcgaaagc aagctga //