BBa_K415202
1
BBa_K415202
cymR Repressor Protein
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
CymR was derived from the organism Pseudomonas Putida. The copy of cymR used in the construction of BBa_K415202 was generously donated by Noah Davidsohn.
The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part
BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR
changes conformation upon induction with cumate (isopropylbenozate), and may be used in a
manner similar to the lacI/IPTG system.
The cymR repressor appears to bind the complementary cmt operator more efficiently than
lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."
true
false
_525_
0
6719
9
Discontinued
false
This repressor required site-directed mutagenesis of nucleotide 132 to a G in order to remove
a PstI cut site.
false
Grant Robinson
BBa_K415202_sequence
1
atgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z