BBa_K415202
1
BBa_K415202
cymR Repressor Protein
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
CymR was derived from the organism Pseudomonas Putida. The copy of cymR used in the construction of BBa_K415202 was generously donated by Noah Davidsohn.
The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part
BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR
changes conformation upon induction with cumate (isopropylbenozate), and may be used in a
manner similar to the lacI/IPTG system.
The cymR repressor appears to bind the complementary cmt operator more efficiently than
lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."
true
false
_525_
0
6719
9
Discontinued
false
This repressor required site-directed mutagenesis of nucleotide 132 to a G in order to remove
a PstI cut site.
false
Grant Robinson
BBa_K415203
1
BBa_K415203
B0031 : cymR (Weak cymR Translation Unit)
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
This part consists of BioBricks BBa_B0031, a weak RBS, and BBa_K415202, the cymR repressor.
This is a translational unit consisting of a weak RBS and the cymR repressor protein; cymR
binds tightly and efficiently to the cmt operator, but changes conformation and detaches from
DNA when it forms a complex with cumate (isopropybenozate). This part allows for weak
translation of mature RNA containing the cymR coding sequence.
true
false
_525_
0
6719
9
Discontinued
false
No design considerations to note.
false
Grant Robinson
component2078440
1
BBa_B0031
component2078441
1
BBa_K415202
annotation2078441
1
BBa_K415202
range2078441
1
21
632
annotation2078440
1
BBa_B0031
range2078440
1
1
14
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_K415202_sequence
1
atgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctag
BBa_K415203_sequence
1
tcacacaggaaacctactagatgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctag
BBa_B0031_sequence
1
tcacacaggaaacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z