BBa_K415200
1
BBa_K415200
Strong Constitutive T5 Promoter with cmt Operator (T5-cmt)
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
This part was synthesized via minigene construction by Mr. Gene; its source is the 2010 paper by Choi, et al., titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."
This is a strong, constitutive partial T5 promoter with the cmt operator appended immediately
afterward. This construct was specified in a recent (as of 2010) paper by Choi, et al., titled, "A
novel, versatile, and tightly regulated expression system for E. Coli strains." In the absence
of cymR, this promoter should function as a strong, constitutive promoter; in the presence of
cymR, this promoter should be repressed with essentially zero leaky transcription.
true
false
_525_
0
6719
9
Discontinued
false
This promoter contains the T5 partial promoter, the sequence of which was derived from the 2010 Choi, et al. paper. Like T7 bacteriophage promoters, this T5 promoter is constitutive and strong; unlike T7 promoters, however, it does not require T7 RNA Polymerase. This means that the T5 promoter could have wide applicability as a strong promoter in Escherichia coli and other organisms.
false
Grant Robinson
annotation2078428
1
cmt Operator
range2078428
1
54
85
annotation2078429
1
T5 Partial Promoter
range2078429
1
1
53
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K415205
1
BBa_K415205
J23117 : B0031 : cymR Repressor Protein [Weak cymR Generator]
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
This part consists of BioBricks BBa_J23117 and BBa_K415203.
This is a copy of a moderately expressed cymR repressor under the control of a weak,
constitutive promoter. This is a modular component for circuit construction and a weak
cymR generator.
true
false
_525_
0
6719
9
Discontinued
false
Note that a weak promoter was chosen in order to follow the construction design of Choi, et
al. in the 2010 paper, "A novel, versatile, and tightly regulated expression system for E. Coli
strains." Previous work by Choi and others in M. Extorquens indicated that overexpression
of cymR was potentially toxic to bacterial cells; although data concerning this toxicity is
unavailable, the researchers managed to develop a tightly expressed cymR switch by placing
cymR under the control of a weak, constitutive promoter for kanamycin resistance. As our
team was unable to clarify the origin of this sequence, we instead selected a weak, constitutive
promoter from the Anderson library.
false
Grant Robinson
component2078448
1
BBa_K415202
component2078447
1
BBa_B0031
component2078445
1
BBa_J23117
annotation2078448
1
BBa_K415202
range2078448
1
64
675
annotation2078445
1
BBa_J23117
range2078445
1
1
35
annotation2078447
1
BBa_B0031
range2078447
1
44
57
BBa_K415202
1
BBa_K415202
cymR Repressor Protein
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
CymR was derived from the organism Pseudomonas Putida. The copy of cymR used in the construction of BBa_K415202 was generously donated by Noah Davidsohn.
The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part
BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR
changes conformation upon induction with cumate (isopropylbenozate), and may be used in a
manner similar to the lacI/IPTG system.
The cymR repressor appears to bind the complementary cmt operator more efficiently than
lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."
true
false
_525_
0
6719
9
Discontinued
false
This repressor required site-directed mutagenesis of nucleotide 132 to a G in order to remove
a PstI cut site.
false
Grant Robinson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K415210
1
BBa_K415210
Temporary cymR Stochastic Trigger
2010-12-15T12:00:00Z
2015-05-08T01:12:27Z
TBD
TBD
false
false
_525_
0
6719
9
Not in stock
false
TBD
false
Grant Robinson
component2115115
1
BBa_K415201
component2115105
1
BBa_K415205
annotation2115105
1
BBa_K415205
range2115105
1
1
675
annotation2115115
1
BBa_K415201
range2115115
1
684
905
BBa_K415201
1
BBa_K415201
B0015 : T5-cmt (Terminator : T5-cmt Promoter)
2010-08-19T11:00:00Z
2015-05-08T01:12:27Z
This part comes from BioBricks BBa_B0015 and BBa_K415200.
This is a terminator followed by a copy of the cmt operator downstream of a partial T5 promoter.
This part is an intermediate part that will could be used in order to ensure leaky expression from
components preceding the T5-cmt promoter does not interfere with the activity of the T5-cmt
promoter or any subsequent parts. This composite part consists of BioBricks BBa_K415200 and
BBa_B0015.
true
false
_525_
0
6719
9
Discontinued
false
No design considerations.
false
Grant Robinson
component2078430
1
BBa_B0010
component2078438
1
BBa_K415200
component2078432
1
BBa_B0012
annotation2078432
1
BBa_B0012
range2078432
1
89
129
annotation2078430
1
BBa_B0010
range2078430
1
1
80
annotation2078438
1
BBa_K415200
range2078438
1
138
222
BBa_J23117
1
BBa_J23117
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K415202_sequence
1
atgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctag
BBa_J23117_sequence
1
ttgacagctagctcagtcctagggattgtgctagc
BBa_K415210_sequence
1
ttgacagctagctcagtcctagggattgtgctagctactagagtcacacaggaaacctactagatgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaacaaacagacaatctggtctgtttgtattat
BBa_K415200_sequence
1
aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaacaaacagacaatctggtctgtttgtattat
BBa_K415201_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaacaaacagacaatctggtctgtttgtattat
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_K415205_sequence
1
ttgacagctagctcagtcctagggattgtgctagctactagagtcacacaggaaacctactagatgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgctgcgggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgctag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z